Browsing by Subject "imaging"
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Item INVESTIGATION OF A LINEAR ANTI-SCATTER GRID WITH NONCONVENTIONAL GEOMETRY AND A NOVEL INTERSPACE MATERIAL FOR MEDICAL X-RAY IMAGING(2022-01-01) Medina, Hector; Topoleski, L. D. Timmie; Mechanical Engineering; Engineering, MechanicalThe objective of this research was to investigate the performance of an anti-scatter grid with a novel interspace material and a nonconventional geometry used in medical x-ray imaging. Anti-scatter grids are used to eliminate scattered radiation that is emitted from the imaged object and that reaches the image receptor. Scattered radiation accumulated in the image receptor degrades the image contrast making it harder to visualize details that might be needed in a diagnosis. The prototype grid, "High-Maintenance Flat Fixed-focus" (HM-FF) grid, developed for this research is comprised of a 2.0 mm interspace width, 0.61 mm absorber thickness, and a height of 38.0 mm. The novelty proposed for the interspace construction was the implementation of a foam spacer for every two absorbers creating a septa pair. The use of the interspace material created an alternating pattern of air and foam interspaces, providing the grid with an increased x-ray transmission due to the low density of the materials used while providing structural support. The nonconventional geometry developed with this research was attributed to the increased height of the grid while maintaining a large grid ratio of 19:1. The thickness of the steel absorbers promoted a lower grid frequency design while maintaining x-ray absorption similar to thinner lead septa. Theoretical simulations narrowed the focus to implementing steel as a suitable lead absorber replacement. The main advantage of using steel was in the ease to handle during the fabrication process, while providing improved structural support for the overall grid assembly. The larger grid geometry was proposed in contrast to where current technology is and suggests that improved materials, for both absorbers and interspaces can significantly impact the performance of a grid. The results of the experiments measured primary transmission (Tp), scatter transmission (Ts) and total transmission (Tt) for three different grids: a conventional grid, a prototype grid called the "50-50" grid, and the focus of this research, the HM-FF grid. The main interest was in the comparison of the conventional grid to the HM-FF grid. The Tp for the conventional grid was 55.18%, while the HM-FF had a Tp of 68.38%. The Ts for the conventional grid was 10.07% and 5.99% for the HM-FF grid. Lastly, the Tt for the conventional and for the HM-FF grids was 19.84% and 18.20%, respectively. The performance values that are of interest to measure how good or bad a grid can be are calculated from those three transmission measurements. Using the latest update to the IEC 60627 standard for grid testing, the Image Improvement Factor (Q), demonstrated that the HM-FF grid was superior compared to the conventional grid with a value of 2.57 over 1.53. The main driver for pursuing this research was to attempt to contribute to improving the quality of life of humans. The prototype HM-FF grid developed in this research suggests that improved quality images are feasible while attempting to reduce the effects of dosage to patients when obtaining radiographs. The increased performance of the prototype grid was due to the larger nonconventional geometry and the implementation of a more x-ray transparent interspace material.Item Probing Biological Systems Using Innovative Electrochemical Sensing and Imaging Platforms Inspired by Biology(2016-01-01) Macazo, Florika Caling; White, Ryan J; Chemistry & Biochemistry; ChemistryElectrochemical sensing and imaging platforms capable of detecting various analytes have attracted considerable attention in bio- and chemical analysis, as quantification and accurate detection of biologically relevant biomolecules may lead to early prognosis of certain diseases. Particularly, the utility of biological agents as biorecognition elements, such as nucleic acids and protein channels, have shown tremendous potential in the development of electrochemical-based biosensors owing to the high sensitivity and innate specificity that nature has already provided them with. In this thesis, the innovative use of nucleic acids and protein channels as biosensing and imaging devices to probe biological systems will be discussed in three parts: 1) in electrochemical DNA-based sensing that uses nucleic acids to monitor biochemical-binding mechanisms such as cooperativity; 2) in protein nanopore-based sensing that employs a ligand-gated, naturally occurring protein channel with specificity for adenosine triphosphate (ATP) to quantitatively detect physiological concentrations of ATP in solution; and 3) in protein channel-based imaging that demonstrates the potential of protein channels to be integrated into as imaging probes, which can be employed in scanning ion conductance microscopy to perform simultaneous topography imaging and specific molecule mapping across a synthetic membrane. In all parts, the development of the methodology is first described followed by its direct implementation in a number of biochemical and bioanalytical applications. The first part of this manuscript describes the use of an oligodeoxythymidylate [(dT)ₙ] electrochemical-DNA (E-DNA) based sensor for the direct monitoring of cooperative and non-cooperative binding of bacteriophage T4 gene 32 protein (g32p), a single-stranded DNA-binding protein that exhibits positive cooperativity when it binds to single-strand DNA (ssDNA). This demonstrates a new ability of E-DNA sensors, which may provide a rapid and simple methodology in understanding DNA-binding protein interactions, and, thus, adds to the growing toolbox enabled by this class of sensors. The second part of the manuscript describes the utility of the pore-forming nature of a ligand-gated heat shock cognate 70 (Hsc70) protein for the direct quantification of ATP. Hsc70 reconstitutes into phospholipid membranes and forms an ATP-regulated channel that exhibits multiple conductance states. The measurement of "charge flux" to characterize the ATP-regulated multi-conductance nature of Hsc70 is utilized as a new method of data analysis in order to achieve reproducible quantitation of ATP. This provides a universal method for quantifying ion-channel activity of proteins for the purpose of building specific and sensitive nanopore-based biosensors. The last part of the manuscript describes the development of a novel, bioinspired scanning ion conductance microscopy (bio-SICM) technique that couples the sensitivity and chemical selectivity of protein channels with the imaging ability of SICM to perform simultaneous pore imaging and specific molecule mapping. The framework of the bio-SICM platform is established using the single-molecule ability of the α-hemolysin (αHL) protein channel to map the spatial localization of β-cyclodextrin (βCD) target molecules crossing a single 25-µm-diameter substrate pore opening. This analytical approach not only demonstrates the potential of protein channels to act as sensing elements in imaging probes, but also extends the utility of SICM by enabling selective chemical imaging of specific target molecules, while simultaneously providing topographical information about the net ion flux through a pore under a concentration gradient. With further optimization, the bio-SICM platform will provide a powerful analytical methodology that is generalizable, and thus, offers significant utility in a myriad of bioanalytical applications.