Browsing by Subject "lung cancer"
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Item EXAMINING GENETIC PROPERTIES AND CELLULAR STRUCTURES OF CLONAL 3D CULTURES CONSTRUCTED FROM PATIENT- DERIVED ORGANOIDS(2020-11-16) Wade, Anna; Boyd, Ann L.; Hood College Department of Biology; Biomedical Science (M.S.)Current anti-cancer drug development studies are progressing towards the use of 3D organoid cell line cultures generated from cancer patient tumors for in vitro models. Establishing clonal 3D culture cell lines in vitro from a patient’s tumor will supply drug trials the type of model containing their target without the additional cells- that differ and may interfere with the results of the drug study. This would precede the tested drug being combined with others for personalized cancer medicine for the patient. Lung cancer, similar to other cancer types, may result from more than one cellular mutation and be a heterogeneous collection of cells. Targeting different cell subpopulations of the tumor is a solution to eradicate the entire cancer. This project will identify the benefits presented by using clonal cancer cell lines generated in 3D culture compared to clones grown in 2D culture through establishing lung cancer clonal 3D cultures in optimal conditions, isolating cell populations found in lung cancer, and testing anti-cancer drug efficacy on 2D and 3D cultures.Item HOW LINEAR ELASTIC MATERIAL PROPERTIES AFFECT LUNG TUMOR MOTION AND DEFORMATION: A FINITE ELEMENT ANALYSIS.(2023-01-01) Ray, Noelle; Topoleski, L.D. Timmie; Mechanical Engineering; Engineering, MechanicalRespiratory motion is a complication for radiation treatment (RT) of lung cancer. A moving and deforming tumor is difficult to radiate with a stationary beam. The tumor will not receive the entire radiation dose and healthy tissue will be exposed to the radiation. Management techniques like surrogate motion models (SMMs) have been developed to help mitigate this problem by using 4-Dimensional CT (4DCT) scans to predict tumor motion during the RT. These SMMs frequently use approximated material property values for the lungs and tumor(s); however, errors caused by using incorrect values are rarely investigated. This work presents an examination of the effect material properties have on tumor movement and deformation using a finite element (FE) model of the lung and tumor in COMSOL. A range of values was assigned to density, Poisson?s ratio, and elastic modulus for the lung and the tumor. Lung density showed no effect on tumor movement or deformation. Displacement differences between material property values ranged from 0.0043- 1.5304cm. The lung elastic modulus has the greatest effect on tumor displacement, with a range of 1.5cm. Lung Poisson?s ratio values affected displacement with a maximum displacement range of 0.1942cm. Tumor elastic modulus and Poisson?s ratio showed insignificant effects on displacement of the tumor. It was concluded that the elastic modulus value for the lung should be a personalized approximation to provide optimal modeling results in SMMs, thereby maximizing the radiation dose to the tumor, minimizing radiation dose to the healthy tissue, and improving the life of the patient.