Browsing by Subject "phosphoenolpyruvate carboxykinase"
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Item Identification and characterization of the pckA gene from Staphylococcus aureus(American Society for Microbiology, 1996-06) Scovill, William H.; Schreier, Harold J.; Bayles, Kenneth W.The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.Item Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant(American Society for Microbiology, 2012-10) Schreier, Harold J.; Dejtisakdi, Wipawee; Escalante, Jose Ortega; Brailo, MarinaA Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.