Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: Characterization of PC12 cell response
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2013-06-22
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Silviya P. Zustiak , Stephanie Pubill , Andreia Ribeiro , Jennie B. Leach, Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: Characterization of PC12 cell response, Biotechnology Progress Volume 29, Issue 5 , 2013, https://doi.org/10.1002/btpr.1761
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"This is the peer reviewed version of the following article: Silviya P. Zustiak , Stephanie Pubill , Andreia Ribeiro , Jennie B. Leach, Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: Characterization of PC12 cell response, Biotechnology Progress Volume 29, Issue 5 , 2013, https://doi.org/10.1002/btpr.1761, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/btpr.1761. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
"This is the peer reviewed version of the following article: Silviya P. Zustiak , Stephanie Pubill , Andreia Ribeiro , Jennie B. Leach, Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: Characterization of PC12 cell response, Biotechnology Progress Volume 29, Issue 5 , 2013, https://doi.org/10.1002/btpr.1761, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/btpr.1761. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Abstract
The central nervous system (CNS) has a low intrinsic potential for regeneration following injury
and disease, yet neural stem/progenitor cell (NPC) transplants show promise to provide a dynamic
therapeutic in this complex tissue environment. Moreover, biomaterial scaffolds may improve the
success of NPC-based therapeutics by promoting cell viability and guiding cell response. We
hypothesized that a hydrogel scaffold could provide a temporary neurogenic environment that
supports cell survival during encapsulation, and degrades completely in a temporally controlled
manner to allow progression of dynamic cellular processes such as neurite extension. We utilized
PC12 cells as a model cell line with an inducible neuronal phenotype to define key properties of
hydrolytically-degradable poly(ethylene glycol) hydrogel scaffolds that impact cell viability and
differentiation following release from the degraded hydrogel. Adhesive peptide ligands (RGDS,
IKVAV or YIGSR), were required to maintain cell viability during encapsulation; as compared to
YIGSR, the RGDS and IKVAV ligands were associated with a higher percentage of PC12 cells
that differentiated to the neuronal phenotype following release from the hydrogel. Moreover,
among the hydrogel properties examined (e.g., ligand type, concentration), total polymer density
within the hydrogel had the most prominent effect on cell viability, with densities above 15% w/v
leading to decreased cell viability likely due to a higher shear modulus. Thus, by identifying key
properties of degradable hydrogels that affect cell viability and differentiation following release
from the hydrogel, we lay the foundation for application of this system towards future applications
of the scaffold as a neural cell delivery vehicle.