Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans
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2018-07-30
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Boppidi, Karthik R. et. al. Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans. Scientific Reports 8, Article number 11433 (2018)
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Attribution 4.0 International (CC BY 4.0)
Abstract
Filamentous fungi are widely used in the production of a variety of industrially relevant enzymes
and proteins as they have the unique ability to secrete tremendous amounts of proteins. However,
the secretory pathways in filamentous fungi are not completely understood. Here, we investigated
the role of a mutation in the POlarity Defective (podB) gene on growth, protein secretion, and cell
wall organization in Aspergillus nidulans using a temperature sensitive (Ts) mutant. At restrictive
temperature, the mutation resulted in lack of biomass accumulation, but led to a significant increase in
specific protein productivity. Proteomic analysis of the secretome showed that the relative abundance
of 584 (out of 747 identified) proteins was altered due to the mutation. Of these, 517 were secreted at
higher levels. Other phenotypic differences observed in the mutant include up-regulation of unfolded
protein response (UPR), deformation of Golgi apparatus and uneven cell wall thickness. Furthermore,
proteomic analysis of cell wall components in the mutant revealed the presence of intracellular
proteins in higher abundance accompanied by lower levels of most cell wall proteins. Taken together,
results from this study suggest the importance of PodB as a target when engineering fungal strains for
enhanced secretion of valuable biomolecules.