Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions
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Date
2014-04-30
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Citation of Original Publication
Nazrul Islam, Yonghyun Kim, Julia M Ross and Mark R Marten, Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions, Proteome Science 2014 , https://doi.org/10.1186/1477-5956-12-21
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Attribution 2.0 Generic (CC BY 2.0)
Attribution 2.0 Generic (CC BY 2.0)
Abstract
Background: The biofilm forming bacterium Staphylococcus aureus is responsible for maladies ranging from severe
skin infection to major diseases such as bacteremia, endocarditis and osteomyelitis. A flow displacement system
was used to grow S. aureus biofilms in four physiologically relevant fluid shear rates (50, 100, 500 and 1000 s−1) to
identify proteins that are associated with biofilm.
Results: Global protein expressions from the membrane and cytosolic fractions of S. aureus biofilm cells grown under
the above shear rate conditions are reported. Sixteen proteins in the membrane-enriched fraction and eight proteins in
the cytosolic fraction showed significantly altered expression (p < 0.05) under increasing fluid shear. These 24 proteins
were identified using nano-LC-ESI-MS/MS. They were found to be associated with various metabolic functions such as
glycolysis / TCA pathways, protein synthesis and stress tolerance. Increased fluid shear stress did not influence the
expression of two important surface binding proteins: fibronectin-binding and collagen-binding proteins.
Conclusions: The reported data suggest that while the general metabolic function of the sessile bacteria is minimal
under high fluid shear stress conditions, they seem to retain the binding capacity to initiate new infections.