A comparison of Lyse-It to other cellular sample preparation, bacterial lysing, and DNA fragmentation technologies
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Author/Creator ORCID
Date
2019-07-23
Type of Work
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Citation of Original Publication
Santaus TM, Li S, Saha L, Chen WH, Bhagat S, Stine OC, et al. (2019) A comparison of Lyse-It to other cellular sample preparation, bacterial lysing, and DNA fragmentation technologies. PLoS ONE 14(7): e0220102. https://doi.org/10.1371/journal.pone.0220102
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Attribution 4.0 International (CC BY 4.0)
Attribution 4.0 International (CC BY 4.0)
Abstract
The ability for safe and rapid pathogenic sample transportation and subsequent detection is
an increasing challenge throughout the world. Herein, we describe and use bead-beating,
vortex, sonication, 903 protein saver cards, and Lyse-It methods, aiming to inactivate Grampositive and -negative bacteria with subsequent genome DNA (quantitative Polymerase
Chain Reaction) qPCR detection. The basic concepts behind the four chosen technologies
is their versatility, cost, and ease of use in developed and underdeveloped countries. The
four methods target the testing of bacterial resilience, cellular extraction from general and
complex media and subsequent DNA extraction for qPCR detection and amplification.
These results demonstrate that conventional high temperature heating, 903 protein saver
cards, and Lyse-It are all viable options for inactivating bacterial growth for safe shipping.
Additionally, Lyse-It was found to be particularly useful as this technology can inactivate
bacteria, extract cells from 903 protein saver cards, lyse bacterial cells, and additionally
keep genomic DNA viable for qPCR detection.