Development and Characterization of a Dither-Based Super-Resolution Reconstruction Method for Fiber Imaging Arrays
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Date
2017-01-01
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Department
Chemistry & Biochemistry
Program
Chemistry
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Access limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission.
Abstract
Chemical imaging is an important tool for providing insight into function, role, and spatial distribution of analytes. This thesis describes the use of imaging fiber bundles (IFB) for super-resolution reconstruction using surface enhanced Raman scattering (SERS) showing improvement in resolution with arrayed bundles for the first time. Additionally this thesis describes characteristics of the IFB with regards to cross-talk as a function of aperture size. The first part of this thesis characterizes the IFB for both tapered and untapered bundles in terms of cross-talk. Cross-talk is defined as the amount of light leaking from a central fiber element in the imaging fiber bundle to surrounding fiber elements. To make this measurement ubiquitous for all imaging bundles, quantum dots were employed. Untapered and tapered IFB possess cross-talk of 2% or less, with fiber elements down to 32nm. The second part of this thesis employs a super resolution reconstruction algorithm using projection onto convex sets for resolution improvement. When using IFB arrays, the point spread function (PSF) of the array can be known accurately if the fiber elements over fill the pixel detector array. Therefore, the use of the known PSF compared to a general blurring kernel was evaluated. Relative increases in resolution of 12% and 2% at the 95% confidence level are found, when compared to a reference image, for the general blurring kernel and PSF, respectively. The third part of this thesis shows for the first time the use of SERS with a dithered IFB array coupled with super-resolution reconstruction. The resolution improvement across a step-edge is shown to be approximately 20% when compared to a reference image. This provides an additional means of increasing the resolution of fiber bundles beyond that of just tapering. Furthermore, this provides a new avenue for nanoscale imaging using these bundles. Lastly, synthetic data with varying degrees of signal-to-noise (S/N) were employed to explore the relationship S/N has with the reconstruction process. It is generally shown that increasing the number images used in the reconstruction process and increasing the S/N will improve the reconstruction providing larger increases in resolution.