Does Docosahexaenoic Acid (DHA) Inhibit Metastasis in B16 Cell Lines by Altering Cell Adhesion Potentials and Cell Motility?
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Date
2018
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Biological Sciences
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Abstract
Docosahexaenoic acid (DHA, an ω-3 fatty acid) has dramatic effects on many cancer cell lines including the ability to reduce their metastatic capacity. Though the details of the mechanisms of many of these effects remain unclear, DHA may alter the adhesion potential and motility, both of which contribute to the overall metastatic capacity of tumor cells. This research tests the hypothesis that DHA reduces metastatic capacity of cells by altering cell adhesion potentials and cell motility. Two isolation variants of the B16 mouse melanoma cell line will be used, one that is aggressively metastatic (B16-F10) and one that is mildly metastatic (B16-F1). Two independent assays were performed. One used a cell-entrapped fluorescent dye to measure the dose-dependent rate of cell adhesion to a substratum. The other microscopically measured dose-dependent changes in the rate of cell movement across the substratum. The results of the adhesion assays showed dramatic, DHA dose-dependent changes in the adhesion parameter, yet inconclusive data for the motility assay. Treatment with DHA reduces the adhesion percentage of both cell lines however, the effects are more pronounced in the more aggressive cell line. These results support the hypothesis that DHA reduces the metastatic potential of B16 cells by altering their ability to adhere to the substratum.