The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control

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dc.contributor.authorZengel, Janice M.
dc.contributor.authorJerauld,  Adam
dc.contributor.authorWalker,  Andre
dc.contributor.authorWahl,  Markus C.
dc.contributor.authorLindahl,  Lasse
dc.date.accessioned2019-06-19T18:05:34Z
dc.date.available2019-06-19T18:05:34Z
dc.date.issued2003-10
dc.description.abstractRibosomal proteins L4 and L22 both have a globular domain that sits on the surface of the large ribosomal subunit and an extended loop that penetrates its core. The tips of both loops contribute to the lining of the peptide exit tunnel and have been implicated in a gating mechanism that might regulate the exit of nascent peptides. Also, the extensions of L4 and L22 contact multiple domains of 23S rRNA, suggesting they might facilitate rRNA folding during ribosome assembly. To learn more about the roles of these extensions, we constructed derivatives of both proteins that lack most of their extended loops. Our analysis of ribosomes carrying L4 or L22 deletion proteins did not detect any significant difference in their sedimentation property or polysome distribution. Also, the role of L4 in autogenous control was not affected. We conclude that these extensions are not required for ribosome assembly or for L4-mediated autogenous control of the S10 operon.en_US
dc.description.sponsorshipThis work was supported by the National Institute of General Medical Science Grant 54876.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1370483/en_US
dc.format.extent10 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m285wt-fgey
dc.identifier.citationJANICE M. ZENGEL, et.al, The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control, RNA. 2003 Oct; 9(10): 1188–1197. DOI: 10.1261/rna.5400703en_US
dc.identifier.urihttps://dx.doi.org/10.1261%2Frna.5400703
dc.identifier.urihttp://hdl.handle.net/11603/14272
dc.language.isoen_USen_US
dc.publisherThe RNA Societyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.rights
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAutogenous controlen_US
dc.subjectpolysomesen_US
dc.subjectprotein structureen_US
dc.subjectrRNAen_US
dc.titleThe extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous controlen_US
dc.typeTexten_US

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