A STUDY OF SURFACE PLASMON-COUPLED EMISSION FROM RHODAMINE 6G USING PICOSECOND PULSES AND CONTINUOUS WAVE LIGHT

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dc.contributor.advisorJohnson, Anthony M
dc.contributor.authorWatts, Shelly C
dc.contributor.departmentPhysics
dc.contributor.programPhysics, Applied
dc.date.accessioned2015-10-14T03:13:05Z
dc.date.available2015-10-14T03:13:05Z
dc.date.issued2009-01-01
dc.description.abstractFluorescence measurements are used in life sciences to provide important information about biomolecules (fluorophores) such as structure and conformational changes by detecting the target molecules on surfaces. Currently, fluorescence measurements are performed using free-space (FS) detection, which are mostly isotropic, resulting in detection of approximately 1% of the total emission. The emission process may be limited by the background fluorescence due to its isotropic nature and, photochemical destruction of the fluorophores. Surface Plasmon-Coupled Emission (SPCE) is a fluorescence technique that has been recently introduced that increases the fluorescence yield. SPCE is based on the interaction of excited-state fluorophores with a nearby metal surface. The fluorophores above the metal surface can couple with the plasmon resonances in the metal, resulting in directional and wavelength-resolved emission. The coupled emission is characterized by a dependence of the emission wavelength on the emission angle. In addition, the emission is horizontally (p) polarized. An advantage of the SPCE over FS signal is the reduction of the background fluorescence signal, since only fluorophores close to the metal surface will couple to the surface plasmons. Picosecond pulses were used to study the SPCE properties of Rhodamine 6G fluorophore on a thin silver film. It is expected that using pulsed laser sources can greatly enhance the SPCE signal over the FS signal. The SPCE signal is 3 times higher that the isotropic FS signal. Thus, SPCE technique under pulsed excitation promises to be an effective tool for fluorescence measurements in investigating the optical properties of biomolecules.
dc.formatapplication/pdf
dc.genretheses
dc.identifierdoi:10.13016/M24680
dc.identifier.other10164
dc.identifier.urihttp://hdl.handle.net/11603/1042
dc.languageen
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Theses and Dissertations Collection
dc.relation.ispartofUMBC Graduate School Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Physics Department Collection
dc.rightsThis item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please see http://aok.lib.umbc.edu/specoll/repro.php or contact Special Collections at speccoll(at)umbc.edu.
dc.sourceOriginal File Name: Watts_umbc_0434M_10164.pdf
dc.subjectFluorophores
dc.subjectRhodamine 6G
dc.subjectSPCE
dc.subjectSurface Plasmon-Coupled Emission
dc.subjectSurface Plasmons
dc.subjectUltrashort pulsed excitation
dc.titleA STUDY OF SURFACE PLASMON-COUPLED EMISSION FROM RHODAMINE 6G USING PICOSECOND PULSES AND CONTINUOUS WAVE LIGHT
dc.typeText
dcterms.accessRightsAccess limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission.

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