Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions

Simple item page
dc.contributor.authorLi, X.
dc.contributor.authorLindahl, L.
dc.contributor.authorZengel, J. M.
dc.date.accessioned2019-06-06T17:11:04Z
dc.date.available2019-06-06T17:11:04Z
dc.date.issued1996-01
dc.description.abstractEscherichia coli ribosomal protein L4 has two functions: it is a structural component of the 50S ribosomal sub-unit and it is a repressor of both transcription and translation of its own transcription unit, the 11-gene S10 operon. Genetic and biochemical studies have suggested that L4 can interact with 23S rRNA as well as with both RNA interactions. However, no significant similarities between its two RNA targets can be found at the primary or secondary structure level. To test if identical determinants of L4 are involved in both ribosome assembly and autogenous control, we have isolated L4 mutants defective in either of these functions and asked if a mutant protein divested of one function is also deficient in the other. Several mutations eliminated autogenous control, but still allowed assembly of the mutant L4 protein into functional ribosomes. Conversely, several mutant L4 proteins that could not be detected in 50S subunits nevertheless could regulate expression of the S10 operon. These results indicate that the L4 determinants required for autogenous regulation and ribosome incorporation are not congruent.en_US
dc.description.sponsorshipThis work was supported by a grant from the National Institute of Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369348/en_US
dc.format.extent14 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2nr0n-9edz
dc.identifier.citationX Li, L Lindahl, and J M Zengel, Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions, RNA. 1996 Jan; 2(1): 24–37, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1369348/en_US
dc.identifier.urihttp://hdl.handle.net/11603/14032
dc.language.isoen_USen_US
dc.publisherThe RNA Societyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectautogenous controlen_US
dc.subjectribosome assemblyen_US
dc.subjecttranscription terminationen_US
dc.subjecttranslationen_US
dc.titleRibosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functionsen_US
dc.typeTexten_US

Files

License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Biological Sciences Department
UMBC Faculty Collection