Examination of a Culturable Microbial Population from the Gastrointestinal Tract of the Wood-Eating Loricariid Catfish Panaque nigrolineatus
| dc.contributor.author | Watts, Joy E. M. | |
| dc.contributor.author | McDonald, Ryan | |
| dc.contributor.author | Daniel, Rachelle | |
| dc.contributor.author | Schreier, Harold J. | |
| dc.date.accessioned | 2019-03-22T14:44:52Z | |
| dc.date.available | 2019-03-22T14:44:52Z | |
| dc.date.issued | 2013-08-19 | |
| dc.description.abstract | Fish play a critical role in nutrient cycling and organic matter flow in aquatic environments. However, little is known about the microbial diversity within the gastrointestinal tracts that may be essential in these degradation activities. Panaque nigrolineatus is a loricariid catfish found in the Neotropics that have a rare dietary strategy of consuming large amounts of woody material in its natural environment. As a consequence, the gastrointestinal (GI) tract of P. nigrolineatus is continually exposed to high levels of cellulose and other recalcitrant wood compounds and is, therefore, an attractive, uncharacterized system to study microbial community diversity. Our previous 16S rRNA gene surveys demonstrated that the GI tract microbial community includes phylotypes having the capacity to degrade cellulose and fix molecular nitrogen. In the present study we verify the presence of a resident microbial community by fluorescence microscopy and focus on the cellulose-degrading members by culture-based and 13C-labeled cellulose DNA stable-isotope probing (SIP) approaches. Analysis of GI tract communities generated from anaerobic microcrystalline cellulose enrichment cultures by 16S rRNA gene analysis revealed phylotypes sharing high sequence similarity to known cellulolytic bacteria including Clostridium, Cellulomonas, Bacteroides,Eubacterium andAeromonas spp. Related bacteria were identified in the SIP community, which also included nitrogen-fixing Azospirillum spp. Our ability to enrich for specialized cellulose-degrading communities suggests that the P. nigrolineatus GI tract provides a favorable environment for this activity and these communities may be involved in providing assimilable carbon under challenging dietary conditions. | en_US |
| dc.description.sponsorship | This work was supported, in part, by the National Science Foundation, award 0801830 (J.E.M.W. and H.J.S.) and Marie Curie FP7-RG 276948 (J.E.M.W). The assistance of Damian Smoot (NSF-REU 0552654), Ashley Kurzweil, Rebecca Kulp (NSF-REU 0552654), Moses Demehin (NSF-REU 0552654), Mickey Dehn and Fan Zhang with various portions of the laboratory work was greatly appreciated. We also thank Jay Nelson for assistance with fish maintenance. | en_US |
| dc.description.uri | https://www.mdpi.com/1424-2818/5/3/641 | en_US |
| dc.format.extent | 16 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2ixym-0jd7 | |
| dc.identifier.citation | Joy E. M. Watts , Ryan McDonald , Rachelle Daniel and Harold J. Schreier, Examination of a Culturable Microbial Population from the Gastrointestinal Tract of the Wood-Eating Loricariid Catfish Panaque nigrolineatus, Diversity 2013, 5(3), 641-656; https://doi.org/10.3390/d5030641 | en_US |
| dc.identifier.uri | https://doi.org/10.3390/d5030641 | |
| dc.identifier.uri | http://hdl.handle.net/11603/13125 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | MDPI | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Department of Marine Biotechnology | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.relation.ispartof | UMBC Biological Sciences Department | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution 4.0 International (CC BY 4.0) | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | microbial diversity | en_US |
| dc.subject | cellulose degradation | en_US |
| dc.subject | enrichment | en_US |
| dc.subject | Panaque | en_US |
| dc.subject | gastrointestinal tract | en_US |
| dc.title | Examination of a Culturable Microbial Population from the Gastrointestinal Tract of the Wood-Eating Loricariid Catfish Panaque nigrolineatus | en_US |
| dc.type | Text | en_US |