Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions

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dc.contributor.authorRaman, Babu
dc.contributor.authorNandakumar, M.P.
dc.contributor.authorMuthuvijayan, Vignesh
dc.contributor.authorMarten, Mark R.
dc.date.accessioned2019-01-31T17:20:07Z
dc.date.available2019-01-31T17:20:07Z
dc.date.issued2005-09-22
dc.description.abstractProteome analysis was used to compare global protein expression changes in Escherichia coli fermentation between exponential and glucose-limited fed-batch phase. Two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry were used to separate and identify 49 proteins showing>2-fold difference in expression. Proteins up regulated during exponential phase include ribonucleotide biosynthesis enzymes and ribosomal recycling factor. Proteins up regulated during fed-batch phase include those involved in high-affinity glucose uptake, transport and degradation of alternate carbon sources and TCA cycle, suggesting an enhanced role of the cycle under glucose- and energy-limited conditions. We report the up regulation of several putative proteins (ytfQ,ygiS,ynaF,yggX,yfeX), not identified in any previous study under carbon-limited conditions.en_US
dc.description.urihttps://www.umbc.edu/proteome/ecoli_fedbatch/ecoli_fedbatch.htmen_US
dc.description.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.20570
dc.format.extent10 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2kjqa-dgel
dc.identifier.citationBabu Raman, M.P. Nandakumar, Vignesh Muthuvijayan, Mark R. Marten, Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions, BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 3, NOVEMBER 5, 2005, DOI: 10.1002/bit.20570en_US
dc.identifier.uri10.1002/bit.20570
dc.identifier.urihttp://hdl.handle.net/11603/12678
dc.language.isoen_USen_US
dc.publisherWiley Periodicalsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rights"This is the peer reviewed version of the following article: Babu Raman, M.P. Nandakumar, Vignesh Muthuvijayan, Mark R. Marten, Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions, BIOTECHNOLOGY AND BIOENGINEERING, VOL. 92, NO. 3, NOVEMBER 5, 2005, DOI: 10.1002/bit.20570, which has been published in final form at https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.20570. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
dc.subjectfed-batch fermentationen_US
dc.subjectproteome analysisen_US
dc.subjectnutrient limitationen_US
dc.subjectstarvationen_US
dc.subjectstationary phaseen_US
dc.subjectEscher-ichia colien_US
dc.titleProteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditionsen_US
dc.typeTexten_US

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