Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein
| dc.contributor.author | Pecher, Wolf T | |
| dc.contributor.author | Kim, Jong-Myoung | |
| dc.contributor.author | Dassarma, Priya | |
| dc.contributor.author | Karan, Ram | |
| dc.contributor.author | Sinnis, Photini | |
| dc.contributor.author | Dassarma, Shiladitya | |
| dc.date.accessioned | 2017-06-23T20:49:57Z | |
| dc.date.available | 2017-06-23T20:49:57Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | We recently developed a novel expression system employing the halophilic Archaeon Halobacterium sp. NRC-1 for scaled-up production and nanoparticle-display of antigenic proteins. Here, we have targeted the major human parasite Plasmodium falciparum circumsporozoite protein (CSP), which is of interest for formulation of a protective malaria vaccine. A codon-optimized synthetic gene coding the full-length CSP was inserted downstream of the strong promoter for gvpA, the major gas vesicle nanoparticle protein gene, in the pDRK expression vector, and as a fusion to the gvpC protein in the pSD expression vector for display on the surface of gas vesicle nanoparticles. We found that the pDRK-CSP expression plasmid programmed high-level production of full-length CSP and the pSD-CSP expression plasmid programmed production of a GvpC-CSP fusion protein, for display on gas vesicle nanoparticles. The Halobacterium sp. expression system provides a novel approach and a potentially valuable technical advancement for the production of P. falciparum CSP for malaria vaccine development. | en_US |
| dc.genre | chapters | en_US |
| dc.identifier | doi:10.13016/M25X25C46 | |
| dc.identifier.citation | Pecher, W. T., Kim, J.-M., DasSarma, P., Karan, R., Sinnis, P., & DasSarma, S. (January 01, 2016). <i>Halobacterium</i> Expression System for Production of Full-Length <i>Plasmodium falciparum</i> Circumsporozoite Protein. | en_US |
| dc.identifier.isbn | 978-3-319-13520-5 978-3-319-13521-2 | |
| dc.identifier.issn | 2367-1017 | |
| dc.identifier.uri | 10.1007/978-3-319-13521-2_25 | |
| dc.identifier.uri | http://hdl.handle.net/11603/4188 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Springer | en_US |
| dc.relation.isAvailableAt | University of Baltimore | |
| dc.title | Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein | en_US |
| dc.type | Text | en_US |