Evaluation of Biological and cDNA Clone-Derived Stocks of Venezuelan Equine Encephalitis Virus TC-83 Vaccine Strain

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dc.contributor.advisorBoyd, Ann
dc.contributor.advisorSmith, Oney
dc.contributor.advisorGlass, Pamela
dc.contributor.authorBall, Matthew
dc.contributor.departmentDepartment of Biologyen_US
dc.contributor.programDepartmental Honorsen_US
dc.date.accessioned2019-04-29T17:37:14Z
dc.date.available2019-04-29T17:37:14Z
dc.date.issued2019-04
dc.description.abstractThe objective of this research study was to evaluate the potential antibody escape mutations identified. The specific aims of this study were as follows: Aim 1: Generate mutant virus stocks containing the identified mutations, singly or in combination; Aim 2: Characterize the mutant viruses, along with the biological or clone-derived wild-type viruses, for fitness and ability to bind antibody 1A3B-7. The results of these studies demonstrated that the biological and clone-derived VEEV TC-83 viruses behaved similarly with regards to replication in cell culture and antibody neutralization. Though there appeared to be some differences in virulence in the C3H/HeN mouse model, the difference in survival was not statistically different. The results of these studies were important to provide the necessary foundation for characterization of the potential antibody escape mutations as the mutants viruses must be clone-derived from cDNA. Future research will observe the two escape mutant viruses with nucleotide changes in the E2 glycoprotein at nucleotide 9177 and 9189. Evaluation of the escape mutants may suggest the most effective way to develop a therapeutic against VEEV would be through the administration of a broadly neutralizing antibody or a cocktail of monoclonal antibodies which recognize multiple epitopes on the surface of the virus particle.en_US
dc.format.extent34 pagesen_US
dc.genreDepartmental Honors Research Paperen_US
dc.identifierdoi:10.13016/m20qgq-ytbs
dc.identifier.urihttp://hdl.handle.net/11603/13523
dc.language.isoen_USen_US
dc.relation.isAvailableAtHood College
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectTC83en_US
dc.subjectcDNA Clone Deriveden_US
dc.subjectVirulenceen_US
dc.subjectReplication Kineticsen_US
dc.subjectAntibody neutralizationen_US
dc.subjectantibody escape mutanten_US
dc.subjectVenezuelan Equine Encephalitis Virusen_US
dc.titleEvaluation of Biological and cDNA Clone-Derived Stocks of Venezuelan Equine Encephalitis Virus TC-83 Vaccine Strainen_US
dc.typeTexten_US

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