Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans

Simple item page
dc.contributor.authorBoppidi, Karthik R.
dc.contributor.authorRibeiro, Liliane Fraga Costa
dc.contributor.authorIambamrung, Sirasa
dc.contributor.authorNelson, Sidney M.
dc.contributor.authorWang, Yan
dc.contributor.authorMomany, Michelle
dc.contributor.authorRichardson, Elizabeth A.
dc.contributor.authorLincoln, Stephen
dc.contributor.authorSrivastava, Ranjan
dc.contributor.authorHarris, Steven D.
dc.contributor.authorMarten, Mark R.
dc.date.accessioned2019-02-11T15:43:13Z
dc.date.available2019-02-11T15:43:13Z
dc.date.issued2018-07-30
dc.description.abstractFilamentous fungi are widely used in the production of a variety of industrially relevant enzymes and proteins as they have the unique ability to secrete tremendous amounts of proteins. However, the secretory pathways in filamentous fungi are not completely understood. Here, we investigated the role of a mutation in the POlarity Defective (podB) gene on growth, protein secretion, and cell wall organization in Aspergillus nidulans using a temperature sensitive (Ts) mutant. At restrictive temperature, the mutation resulted in lack of biomass accumulation, but led to a significant increase in specific protein productivity. Proteomic analysis of the secretome showed that the relative abundance of 584 (out of 747 identified) proteins was altered due to the mutation. Of these, 517 were secreted at higher levels. Other phenotypic differences observed in the mutant include up-regulation of unfolded protein response (UPR), deformation of Golgi apparatus and uneven cell wall thickness. Furthermore, proteomic analysis of cell wall components in the mutant revealed the presence of intracellular proteins in higher abundance accompanied by lower levels of most cell wall proteins. Taken together, results from this study suggest the importance of PodB as a target when engineering fungal strains for enhanced secretion of valuable biomolecules.en_US
dc.description.sponsorshipThis material is based upon work supported by the National Science Foundation under Grant No. 1517309.en_US
dc.description.urihttps://www.nature.com/articles/s41598-018-29615-zen_US
dc.format.extent11 pagesen_US
dc.genrejournal aritclesen_US
dc.identifierdoi:10.13016/m24hxz-kumn
dc.identifier.citationKarthik R. Boppidi, Liliane Fraga Costa Ribeiro, Sirasa Iambamrung, Sidney M. Nelson, Yan Wang, Michelle Momany, Elizabeth A. Richardson, Stephen Lincoln, Ranjan Srivastava, Steven D. Harris & Mark R. Marten, Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans, Scientific Reportsvolume 8, Article number: 11433 (2018) , DOI : https://doi.org/10.1038/s41598-018-29615-zen_US
dc.identifier.urihttps://doi.org/10.1038/s41598-018-29615-z
dc.identifier.urihttp://hdl.handle.net/11603/12757
dc.language.isoen_USen_US
dc.publisherSpringer Nature Publishing AG.en_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectsecretion patternsen_US
dc.subjectcell wallen_US
dc.subjectpodb functionen_US
dc.subjectfungusen_US
dc.subjectaspergillus nidulansen_US
dc.titleAltered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulansen_US
dc.typeTexten_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41598-018-29615-z.pdf
Size:
2.19 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Chemical, Biochemical & Environmental Engineering Department
UMBC Faculty Collection