The problem of genetic code misreading during protein synthesis

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dc.contributor.authorJoshi, Kartikeya
dc.contributor.authorCao, Ling
dc.contributor.authorFarabaugh, Philip J.
dc.date.accessioned2019-02-04T16:02:07Z
dc.date.available2019-02-04T16:02:07Z
dc.date.issued2018-12-17
dc.description.abstractSaccharomyces cerevisiae has been an important model for determining the frequency of translational misreading events, those in which a tRNA pairs incorrectly to the mRNA and inserts an amino acid not specified by the codon in the mRNA. Misreading errors have been quantified in vivo using reporter protein systems or mass spectrom- etry with both approaches converging on a simple model for most misreading. The available data show that misreading tRNAs must form stereotypical base mismatches that correspond to those that can mimic Watson–Crick base pairs when formed in the ribosomal A site. Errors involving other mismatches occur significantly less frequently. This work debunks the idea of an average misreading frequency of 5 × 10 −4 per codon that extends across the genetic code. Instead, errors come in two distinct classes— high frequency and low frequency events—with most errors being of the low frequency type. A comparison of misreading errors in S. cerevisiae and Escherichia coli suggests the existence of a mechanism that reduces misreading frequency in yeast; this mechanism may operate in eukaryotes generally.en_US
dc.description.sponsorshipNational Institutes of Health, Grant/Award Number: GM 029480; National Science Foundation, Grant/Award Number: 1645795en_US
dc.description.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/yea.3374en_US
dc.format.extent8 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m241oa-unql
dc.identifier.citationKartikeya Joshi , Ling Cao, Philip J. Farabaugh, The problem of genetic code misreading during protein synthesis, Yeast, Volume 36, Issue 1, 2018, https://doi.org/10.1002/yea.3374en_US
dc.identifier.urihttps://doi.org/10.1002/yea.3374
dc.identifier.urihttp://hdl.handle.net/11603/12695
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsThis is the peer reviewed version of the following article: Kartikeya Joshi , Ling Cao, Philip J. Farabaugh, The problem of genetic code misreading during protein synthesis, Yeast, Volume 36, Issue 1, 2018, https://doi.org/10.1002/yea.3374, which has been published in final form at https://onlinelibrary.wiley.com/doi/epdf/10.1002/yea.3374. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
dc.rightsAccess to this item will begin on December 17. 2019
dc.subjectsaccharomycesen_US
dc.subjecttranslational erroren_US
dc.subjectmisreadingen_US
dc.subjecttRNA modificationen_US
dc.titleThe problem of genetic code misreading during protein synthesisen_US
dc.typeTexten_US

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