Repressed synthesis of ribosomal proteins generates protein-specific cell cycle and morphological phenotypes
| dc.contributor | Weis, Karsten | |
| dc.contributor.author | Thapa, Mamata | |
| dc.contributor.author | Bommakanti, Ananth | |
| dc.contributor.author | Shamsuzzaman, Md. | |
| dc.contributor.author | Gregory, Brian | |
| dc.contributor.author | Samsel, Leigh | |
| dc.contributor.author | Zengel, Janice M. | |
| dc.contributor.author | Lindahl, Lasse | |
| dc.date.accessioned | 2019-06-20T17:21:25Z | |
| dc.date.available | 2019-06-20T17:21:25Z | |
| dc.date.issued | 2013-10-09 | |
| dc.description.abstract | The biogenesis of ribosomes is coordinated with cell growth and proliferation. Distortion of the coordinated synthesis of ribosomal components affects not only ribosome formation, but also cell fate. However, the connection between ribosome biogenesis and cell fate is not well understood. To establish a model system for inquiries into these processes, we systematically analyzed cell cycle progression, cell morphology, and bud site selection after repression of 54 individual ribosomal protein (r-protein) genes in Saccharomyces cerevisiae. We found that repression of nine 60S r-protein genes results in arrest in the G2/M phase, whereas repression of nine other 60S and 22 40S r-protein genes causes arrest in the G1 phase. Furthermore, bud morphology changes after repression of some r-protein genes. For example, very elongated buds form after repression of seven 60S r-protein genes. These genes overlap with, but are not identical to, those causing the G2/M cell cycle phenotype. Finally, repression of most r-protein genes results in changed sites of bud formation. Strikingly, the r-proteins whose repression generates similar effects on cell cycle progression cluster in the ribosome physical structure, suggesting that different topological areas of the precursor and/or mature ribosome are mechanistically connected to separate aspects of the cell cycle. | en_US |
| dc.description.sponsorship | This work was supported by National Science Foundation Grants MCB0349443 and 0920578 to J.M.Z. and L.L. and National Science Foundation Major Research Instrumentation Grant DBI-0722569 to D.B. and T.G. Finally, we acknowledge the use of the UCSF Chimera Package supported by National Institute of General Medical Sciences Grant P41-GM103311. | en_US |
| dc.description.uri | https://www.molbiolcell.org/doi/10.1091/mbc.e13-02-0097 | en_US |
| dc.format.extent | 14 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2notm-9tuf | |
| dc.identifier.citation | Mamata Thapa, et.al, Repressed synthesis of ribosomal proteins generates protein-specific cell cycle and morphological phenotypes, Molecular Biology of the CellVol. 24, No. 23, https://doi.org/10.1091/mbc.e13-02-0097 | en_US |
| dc.identifier.uri | https://doi.org/10.1091/mbc.e13-02-0097 | |
| dc.identifier.uri | http://hdl.handle.net/11603/14278 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | American Society for Cell Biology | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/3.0/ | |
| dc.subject | ribosomal proteins | en_US |
| dc.subject | protein-specific cell cycle | en_US |
| dc.subject | morphological phenotypes | en_US |
| dc.subject | Saccharomyces cerevisiae | en_US |
| dc.title | Repressed synthesis of ribosomal proteins generates protein-specific cell cycle and morphological phenotypes | en_US |
| dc.type | Text | en_US |