Translational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia coli

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dc.contributor.authorLindahl, L.
dc.contributor.authorArcher, R. H.
dc.contributor.authorMcCormick, J. R.
dc.contributor.authorFreedman, L. P.
dc.contributor.authorZengel, J. M.
dc.date.accessioned2019-06-05T18:05:14Z
dc.date.available2019-06-05T18:05:14Z
dc.date.issued1989-05
dc.description.abstractWe have examined the translational coupling between the first two genes in the S10 ribosomal protein operon. We isolated mutations blocking the translation of the first gene of the operon, coding for S10, and monitored their effects on translation of the downstream gene, coding for L3. All of the mutations inhibiting S10 synthesis also affected the synthesis of L3. However, these experiments were complicated by decreased mRNA synthesis resulting from transcription polarity, which we could only partially eliminate by using a rho-100 strain. To completely eliminate the problem of transcription polarity and obtain a more accurate measurement of the coupling, we replaced the natural S10 promoter with a promoter used by the bacteriophage T7 RNA polymerase. As expected, the T7 RNA polymerase was not subject to transcription polarity. Using this system, we were able to show that a complete abolishment of S10 translation resulted in an 80% inhibition of L3 synthesis. Other experiments show that the synthesis of L3 goes up as a function of increasing S10 synthesis, but the translational coupling does not assure strictly proportional output from the two genes.en_US
dc.description.sponsorshipThis work was supported by a research grant and a Research Career Development Award to L.L. from the National Institute of Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/2651412en_US
dc.format.extent7 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2w7b6-n13x
dc.identifier.citationL Lindahl, R H Archer, J R McCormick, L P Freedman, and J M Zengel, Translational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia coli, J Bacteriol. 1989 May; 171(5): 2639–2645, DOI: 10.1128/jb.171.5.2639-2645.1989en_US
dc.identifier.urihttps://doi.org/10.1128/jb.171.5.2639-2645.1989
dc.identifier.urihttp://hdl.handle.net/11603/14021
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectribosomal protein operonen_US
dc.subjectEscherichia colien_US
dc.subjectRNA polymeraseen_US
dc.subjecttranslational couplingen_US
dc.titleTranslational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia colien_US
dc.typeTexten_US

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