A hairpin structure upstream of the terminator hairpin required for ribosomal protein L4-mediated attenuation control of the S10 operon of Escherichia coli

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dc.contributor.authorZengel, J. M.
dc.contributor.authorLindahl, L.
dc.date.accessioned2019-06-06T17:26:29Z
dc.date.available2019-06-06T17:26:29Z
dc.date.issued1996-04
dc.description.abstractRibosomal protein L4 of Escherichia coli regulates transcription of the 11-gene S1O operon by promoting premature termination of transcription (attenuation) at a specific site within the 172-base untranslated leader. We have analyzed the roles of various domains of the leader RNA in this transcription control. Our results indicate that the first 60 bases of the leader, forming the three proximal hairpin structures, are not essential for in vivo L4-mediated attenuation control. However, a deletion removing the fourth hairpin, which is immediately upstream of the terminator hairpin, eliminates L4's effect on transcription. Base changes disrupting complementarity in the 6-bp stem of this hairpin also abolish L4 control, but compensatory base changes that restore complementarity also restore L4's effect. In vitro transcription studies confirm that this hairpin structure is necessary for L4's role in stimulating transcription termination by RNA polymerase.en_US
dc.description.sponsorshipThis work was supported by a grant from the National Institute for Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC177949/en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m21oi0-qal8
dc.identifier.citationJ M Zengel and L Lindahl, A hairpin structure upstream of the terminator hairpin required for ribosomal protein L4-mediated attenuation control of the S10 operon of Escherichia coli, J Bacteriol. 1996 Apr; 178(8): 2383–2387, https://doi.org/10.1128/jb.178.8.2383-2387.1996en_US
dc.identifier.urihttps://doi.org/10.1128/jb.178.8.2383-2387.1996
dc.identifier.urihttp://hdl.handle.net/11603/14033
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectRNA polymeraseen_US
dc.subjecttranscription translationen_US
dc.subjectribosomal proteinen_US
dc.subjectEscherichia colien_US
dc.titleA hairpin structure upstream of the terminator hairpin required for ribosomal protein L4-mediated attenuation control of the S10 operon of Escherichia colien_US
dc.typeTexten_US

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