Small-angle X-ray scattering characterization of a β-amyloid model in phantoms
| dc.contributor.author | Breedlove, Sophya | |
| dc.contributor.author | Crentsil, Jasson | |
| dc.contributor.author | Dahal, Eshan | |
| dc.contributor.author | Badano, Aldo | |
| dc.date.accessioned | 2020-04-10T18:40:56Z | |
| dc.date.available | 2020-04-10T18:40:56Z | |
| dc.description.abstract | Objective We present a method to prepare an amyloid model at scalable quantities for phantom studies to evaluate small-angle x-ray scattering systems for amyloid detection. Two amyloid models were made from a plasma protein with and without heating. Both models mimic the β-sheet structure of the β-amyloid (βA) plaques in Alzheimer’s disease. Amyloid detection is based on the distinct peaks in the scattering signature of the β-sheet structure. We characterized the amyloid models using a spectral small-angle x-ray scattering (sSAXS) prototype with samples in a plastic syringe and within a cylindrical polymethyl methacrylate (PMMA) phantom. Results sSAXS data show that we can detect the scattering peaks characteristic of amyloid β-sheet structure in both models around 6 and 13 nm⁻¹. The βA model prepared without heating provides a stronger signal in the PMMA phantom. The methods described can be used to prepare models in sufficiently large quantities and used in samples with different packing density to assess the performance of βA quantification systems. | en_US |
| dc.description.sponsorship | SB and JC acknowledge support through the National Science Foundation REU program, EEC Award #1757745. ED acknowledges funding by appointments to the Research Participation Program at the Center for Devices and Radiological Health administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the US Department of Energy and the US Food and Drug Administration (FDA). | en_US |
| dc.description.uri | https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-04969-8 | en_US |
| dc.format.extent | 5 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m27g5m-acid | |
| dc.identifier.citation | Breedlove, Sophya; Crentsil, Jasson; Dahal, Eshan; Badano, Aldo; Small-angle X-ray scattering characterization of a β-amyloid model in phantoms; BMC Research Notes 13,128 (2020); https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-04969-8 | en_US |
| dc.identifier.uri | https://doi.org/10.1186/s13104-020-04969-8 | |
| dc.identifier.uri | http://hdl.handle.net/11603/17987 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | BioMed Central | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Student Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Public Domain Mark 1.0 | * |
| dc.rights | This work was written as part of one of the author's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law. | |
| dc.rights.uri | http://creativecommons.org/publicdomain/mark/1.0/ | * |
| dc.title | Small-angle X-ray scattering characterization of a β-amyloid model in phantoms | en_US |
| dc.type | Text | en_US |