Production of reproductively sterile fish by a non-transgenic gene silencing technology

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dc.contributor.authorWong, Ten-Tsao
dc.contributor.authorZohar, Yonathan
dc.date.accessioned2019-10-31T15:30:50Z
dc.date.available2019-10-31T15:30:50Z
dc.date.issued2015-10-29
dc.description.abstractWe developed a novel bath-immersion technology to produce large numbers of infertile fish. As seafood consumption shifts from fishery harvests towards artificially propagated species, optimization of aquaculture practices will be necessary to maximize food production and minimize ecological impact. Farming infertile fish is the most effective genetic-containment strategy to support the development of environmentally-responsible aquaculture. We discovered that a molecular transporter, Vivo, can effectively carry the Morpholino oligomer (MO) across the chorion, enter the embryo and reach target cells. Vivo-conjugated MO against zebrafish deadend (dnd-MO-Vivo) effectively caused primordial germ cell mis-migration and differentiation into somatic cells, which resulted in generation of infertile fish. Optimal conditions were achieved when embryos, immediately after fertilization, were immersed with dnd-MO-Vivo at the initial concentration of either 60 or 40 μM followed by a lower serially diluted concentration. Under these conditions, 100% induced sterility was achieved even when the total immersion time was reduced from 24 to 5 hours. In 8 independent experiments, 736 adults developed from these conditions were all found to be infertile fish that possessed minimally-developed gonads that lacked any gametes. The results demonstrate that dnd-MO-Vivo bath immersion is an effective strategy to produce infertile fish without introducing transgenic modifications.en_US
dc.description.sponsorshipThe authors thank EuroPharma LLC and the Gudelsky Foundation for supporting this research and publication of the results, the Aquaculture Research Center staff for daily maintenance and health monitoring of experimental animals, and John Stubblefield for editing this manuscript
dc.description.urihttps://www.nature.com/articles/srep15822en_US
dc.format.extent8 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m24hzt-eein
dc.identifier.citationWong, Ten-Tsao; Zohar, Yonathan; Production of reproductively sterile fish by a non-transgenic gene silencing technology; Scientific Reports 5,15822; https://doi.org/10.1038/srep15822en_US
dc.identifier.urihttps://doi.org/10.1038/srep15822
dc.identifier.urihttp://hdl.handle.net/11603/16008
dc.language.isoen_USen_US
dc.publisherNatureen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAnimal biotechnologyen_US
dc.subjectEnvironmental biotechnologyen_US
dc.titleProduction of reproductively sterile fish by a non-transgenic gene silencing technologyen_US
dc.typeTexten_US

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