The RNA of RNase MRP is required for normal processing of ribosomal RNA

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dc.contributor.authorChu, S.
dc.contributor.authorArcher, R. H.
dc.contributor.authorZengel, J. M.
dc.contributor.authorLindahl, L.
dc.date.accessioned2019-06-06T16:50:28Z
dc.date.available2019-06-06T16:50:28Z
dc.date.issued1994-01-18
dc.description.abstractWe have isolated clones which complement the temperature sensitivity and abnormal rRNA processing pattern of the rrp2-2 mutant of Saccharomyces cerevisiae we previously described. DNA sequencing and restriction analysis demonstrated that all clones contain the NME1 gene encoding the RNA of the ribonucleprotein particle RNase MRP. Deletion analysis showed that the NME1 gene is responsible for the complementation of the rrp2-2 phenotype. A single base change was identified in the nme1 gene in the rrp2 mutant, confirming that the RRP2 and NME1 genes are identical. Our experiments therefore indicate that RNase MRP, in addition to its previously reported role in formation of RNA primers for mitochondrial DNA replication [Clayton, D. A. (1991) Trends Biochem. Sci. 16, 107-111], is involved in rRNA processing.en_US
dc.description.sponsorshipThis work was supported by a grant from the National Institute of Allergy and Infectious Diseases.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC43008/en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2kau2-li6o
dc.identifier.citationS Chu, R H Archer, J M Zengel, and L Lindahl, The RNA of RNase MRP is required for normal processing of ribosomal RNA, Proc Natl Acad Sci U S A. 1994 Jan 18; 91(2): 659–663. DOI: 10.1073/pnas.91.2.659en_US
dc.identifier.urihttps://dx.doi.org/10.1073%2Fpnas.91.2.659
dc.identifier.urihttp://hdl.handle.net/11603/14030
dc.language.isoen_USen_US
dc.publisherNational Academy of Sciencesen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Biological Sciences Department Collection
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjecttemperature sensitivityen_US
dc.subjectrrp2-2 mutanten_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectribosomal RNAen_US
dc.titleThe RNA of RNase MRP is required for normal processing of ribosomal RNAen_US
dc.typeTexten_US

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