Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds
| dc.contributor.author | Acosta, Miguel A. | |
| dc.contributor.author | Ymele-Leki, Patrick | |
| dc.contributor.author | Kostov, Yordan V. | |
| dc.contributor.author | Leach, Jennie B. | |
| dc.date.accessioned | 2018-12-17T18:11:46Z | |
| dc.date.available | 2018-12-17T18:11:46Z | |
| dc.date.issued | 2009-03-14 | |
| dc.description.abstract | We present the development and characterization of fluorescent oxygen-sensing microparticles designed for measuring oxygen concentration in microenvironments existing within standard cell culture and transparent three-dimensional (3D) cell scaffolds. The microparticle synthesis employs poly(dimethylsiloxane) to encapsulate silica gel particles bound with an oxygen-sensitive luminophore as well as a reference or normalization fluorophore that is insensitive to oxygen. We developed a rapid, automated and non-invasive sensor analysis method based on fluorescence microscopy to measure oxygen concentration in a hydrogel scaffold. We demonstrate that the microparticles are non-cytotoxic and that their response is comparable to that of a traditional dissolved oxygen meter. Microparticle size (5–40 μm) was selected for microscale-mapping of oxygen concentration to allow measurements local to individual cells. Two methods of calibration were evaluated and revealed that the sensor system enables characterization of a range of hypoxic to hyperoxic conditions relevant to cell and tissue biology (i.e., pO₂ 10–160 mm Hg). The calibration analysis also revealed that the microparticles have a high fraction of quenched luminophore (0.90 ± 0.02), indicating that the reported approach provides significant advantages for sensor performance. This study thus reports a versatile oxygen-sensing technology that enables future correlations of local oxygen concentration with individual cell response in cultured engineered tissues. | en_US |
| dc.description.sponsorship | This work was supported by the Henry Luce Foundation and UMBC. The Leica TCS SP5 confocal microscope was funded by NSF DBI-0722569. | en_US |
| dc.description.uri | https://www.sciencedirect.com/science/article/pii/S0142961209001811?via%3Dihub | en_US |
| dc.format.extent | 16 pages | en_US |
| dc.genre | journal articles postprints | en_US |
| dc.identifier | doi:10.13016/M2XD0R25K | |
| dc.identifier.citation | Miguel A. Acosta, Patrick Ymele-Leki, Yordan V. Kostov, Jennie B. Leach, Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds, Biomaterials Volume 30, Issue 17, June 2009, Pages 3068-3074, https://doi.org/10.1016/j.biomaterials.2009.02.021 | en_US |
| dc.identifier.uri | https://doi.org/10.1016/j.biomaterials.2009.02.021 | |
| dc.identifier.uri | http://hdl.handle.net/11603/12282 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier B.V | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.subject | tissue engineering | en_US |
| dc.subject | hypoxia | en_US |
| dc.subject | optical sensors | en_US |
| dc.subject | microspheres | en_US |
| dc.subject | poly(dimethylsiloxane) | en_US |
| dc.title | Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds | en_US |
| dc.type | Text | en_US |