DEVELOPMENT OF A REAL-TIME PCR ASSAY FOR MOUSE FUR MITES AND EVALUATION OF PILOT STUDY
| dc.contributor.advisor | Hsieh, Wang-Ting | |
| dc.contributor.advisor | Boyd, Ann | |
| dc.contributor.advisor | Laufer, Craig | |
| dc.contributor.author | Poore, Allison | |
| dc.contributor.department | Biomedial Science | en_US |
| dc.contributor.program | Hood College Graduate School Biomedical Science Program | en_US |
| dc.date.accessioned | 2019-03-22T17:37:40Z | |
| dc.date.available | 2019-03-22T17:37:40Z | |
| dc.date.issued | 2019-03-22 | |
| dc.description.abstract | Mouse fur mites Myobia musculi and Myocoptes musculinus are common parasites of mice and present a unique challenge for exclusion from laboratory animal facilities. In this study, a real-time PCR test was developed to detect both mouse fur mites using distinct dye in a single reaction well. Confirmatory endpoint PCR tests were also designed, to confirm equivocal results. The assays were then tested during a pilot study, where known positive animals were used to evaluate the accuracy of environmental targets for screening. In all cages were mice were positive for the fur mite real-time PCR, environmental targets were also positive within 10 days of placement in a new, clean cage. This study shows that the real-time PCR assay and environmental targets are an effective combination for screening of mouse fur mites in laboratory animal facilities. | en_US |
| dc.format.extent | 67 pages | en_US |
| dc.genre | Thesis | en_US |
| dc.identifier | doi:10.13016/m2jehr-byvh | |
| dc.identifier.uri | http://hdl.handle.net/11603/13129 | |
| dc.language.iso | en_US | en_US |
| dc.relation.isAvailableAt | Hood College | |
| dc.subject | Biology, Molecular (0307) | en_US |
| dc.title | DEVELOPMENT OF A REAL-TIME PCR ASSAY FOR MOUSE FUR MITES AND EVALUATION OF PILOT STUDY | en_US |
| dc.type | Text | en_US |