Biocompatible hydrogel membranes for the protection of RNA aptamer-based electrochemical sensors
| dc.contributor.author | Schoukroun-Barnes, Lauren R. | |
| dc.contributor.author | Wagan, Samiullah | |
| dc.contributor.author | Liu, Juan | |
| dc.contributor.author | Leach, Jennie B. | |
| dc.contributor.author | White, Ryan J. | |
| dc.date.accessioned | 2018-12-20T19:34:05Z | |
| dc.date.available | 2018-12-20T19:34:05Z | |
| dc.date.issued | 2013-05-31 | |
| dc.description.abstract | Electrochemical-aptamer based (E-AB) sensors represent a universal specific, selective, and sensitive sensing platform for the detection of small molecule targets. Their specific detection abilities are afforded by oligonucleotide (RNA or DNA) aptamers employed as electrode-bound biorecognition elements. Sensor signaling is predicated on bindinginduced changes in conformation and/or flexibility of the aptamer that is readily measurable electrochemically. While sensors fabricated using DNA aptamers can achieve specific and selective detection even in unadulterated sample matrices, such as blood serum, RNA-based sensors fail when challenged in the same sample matrix without significant sample pretreatment. This failure is at least partially a result of enzymatic degradation of the RNA sensing element. This degradation destroys the sensing aptamer inhibiting the quantitative measurement of the target analyte and thus limits the application of E-AB sensors constructed with RNA aptamer. To circumvent this, we demonstrate that a biocompatible hydrogel membrane protects the RNA aptamer sensor surface from enzymatic degradation for at least 3 hours - a remarkable improvement over the rapid (~minutes) degradation of unprotected sensors. To demonstrate this, we characterize the response of sensors fabricated with representative DNA and RNA aptamers directed against the aminoglycoside antibiotic, tobramycin in blood serum both protected and unprotected by a polyacrylamide membrane. Furthermore, we find encapsulation of the sensor surface with the hydrogel does not significantly impede the detection ability of aptamer-based sensors. This hydrogel-aptamer interface will thus likely prove useful for the long-term monitoring of therapeutics in complex biological media | en_US |
| dc.description.sponsorship | This work was supported by UMBC Startup. | en_US |
| dc.description.uri | https://www.spiedigitallibrary.org/conference-proceedings-of-spie/8719/1/Biocompatible-hydrogel-membranes-for-the-protection-of-RNA-aptamer-based/10.1117/12.2016020.full?SSO=1 | en_US |
| dc.format.extent | 9 pages | en_US |
| dc.genre | conference papers and proceedings | en_US |
| dc.identifier | doi:10.13016/m2s1ck-at0z | |
| dc.identifier.citation | Lauren R. Schoukroun-Barnes, Samiullah Wagan, Juan Liu, Jennie B. Leach, Ryan J. White, Biocompatible hydrogel membranes for the protection of RNA aptamer-based electrochemical sensors , Proceedings Volume 8719, Smart Biomedical and Physiological Sensor Technology X; 87190I (2013) https://doi.org/10.1117/12.2016020 | en_US |
| dc.identifier.uri | https://doi.org/10.1117/12.2016020 | |
| dc.identifier.uri | http://hdl.handle.net/11603/12339 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | SPIE | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. | |
| dc.subject | biosensor | en_US |
| dc.subject | electrochemical detection | en_US |
| dc.subject | biocompatible | en_US |
| dc.subject | aptamers | en_US |
| dc.subject | small molecule detection | en_US |
| dc.subject | serum | en_US |
| dc.title | Biocompatible hydrogel membranes for the protection of RNA aptamer-based electrochemical sensors | en_US |
| dc.type | Text | en_US |