DEVELOPMENT OF A CELL CULTURE, SCREENING, AND SEQUENCING METHOD FOR HIGH-THROUGHPUT ISOLATION OF HIV-POSITIVE CD4+ T-CELLS FROM HIV-INFECTED PATIENTS
| dc.contributor.advisor | Sylvain Laverdure, PhD | |
| dc.contributor.author | Whitney Bruchey | |
| dc.contributor.department | Hood College Biology | |
| dc.contributor.program | Hood College Biomedical and Environmental Science | |
| dc.date.accessioned | 2024-04-26T20:04:49Z | |
| dc.date.available | 2024-04-26T20:04:49Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Human immunodeficiency virus type 1 (HIV-1) is a retrovirus that targets immune cells critical to innate and adaptive immune responses in infected patients. Despite viremia suppression by antiretroviral drug treatments, chronic immune activation persists in HIV-1-infected patients, increasing their risk of HIV-associated chronic comorbidities. Defective HIV-1 proviruses harboring genetic mutations may contribute to persistent immune activation in suppressed HIV-1-infected patients through expression of canonical or novel viral proteins. In this study, we presented the steps taken to develop optimal culture conditions for CD4+ T cells from suppressed HIV-infected patients, and an unbiased nested polymerase chain reaction (PCR) approach for screening of HIV-1 positive cells. Of the 507 potential HIV-1 positive clones detected, we chose three for further bioinformatic analysis that resulted in observation of two novel open reading frames within HIV-1 integrase and reverse transcriptase genes. | |
| dc.format.extent | 57 pages | |
| dc.genre | Master’s thesis | |
| dc.identifier | doi:10.13016/m2ale8-blkg | |
| dc.identifier.uri | http://hdl.handle.net/11603/33319 | |
| dc.language.iso | en_US | |
| dc.rights | Attribution 3.0 United States | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | |
| dc.subject | HIV/AIDS | |
| dc.title | DEVELOPMENT OF A CELL CULTURE, SCREENING, AND SEQUENCING METHOD FOR HIGH-THROUGHPUT ISOLATION OF HIV-POSITIVE CD4+ T-CELLS FROM HIV-INFECTED PATIENTS | |
| dc.type | Text |