Anaerobic Ammonia-Oxidizing Bacteria and Related Activity in Baltimore Inner Harbor Sediment

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dc.contributor.authorTal, Yossi
dc.contributor.authorWatts, Joy E. M.
dc.contributor.authorSchreier, Harold J.
dc.date.accessioned2019-03-22T14:08:55Z
dc.date.available2019-03-22T14:08:55Z
dc.date.issued2005-04
dc.description.abstractThe discovery of bacteria capable of anaerobic ammonia oxidation (anammox) has generated interest in understanding the activity, diversity, and distribution of these bacteria in the environment. In this study anammox activity in sediment samples obtained from the Inner Harbor of Baltimore, Md., was detected by (15)N tracer assays. Anammox-specific oligonucleotide primer sets were used to screen a Planctomycetales-specific 16S rRNA gene library generated from sediment DNA preparations, and four new anammox bacterial sequences were identified. Three of these sequences form a cohesive new branch of the anammox group, and the fourth sequence branches separately from this group. Denaturing gradient gel electrophoresis analysis of sediment incubated with anammox-specific media confirmed the presence of the four anammox-related 16S rRNA gene sequences. Evidence for the presence of anammox bacteria in Inner Harbor sediment was also obtained by using an anammox-specific probe in fluorescence in situ hybridization studies. To our knowledge, this is the first report of anammox activity and related bacterial 16S rRNA gene sequences from the Chesapeake Bay basin area, and the results suggest that this pathway plays an important role in the nitrogen cycle of this estuarine environment. Furthermore, the presence of these bacteria and their activity in sediment strengthen the contention that anammox-related Plactomycetales are globally distributed.en_US
dc.description.sponsorshipYossi Tal was supported by United States-Israel Binational Agricultural Research and Development Fund postdoctoral fellowship FI-305-200. This work was supported in part by research grant IS-3424-03 from the United States-Israel Binational Agricultural Research and Development Fund and by the Living Marine Resources Cooperative Sciences Center of the U.S. NOAA Educational Partnership Program.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/15812006en_US
dc.format.extent6 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2kdvs-v7a4
dc.identifier.citationYossi Tal, Joy E. M. Watts, Harold J. Schreier, Anaerobic Ammonia-Oxidizing Bacteria and Related Activity in Baltimore Inner Harbor Sediment, Appl Environ Microbiol. 2005 Apr;71(4):1816-21, DOI: 10.1128/AEM.71.4.1816-1821.2005en_US
dc.identifier.urihttps://doi.org/10.1128/AEM.71.4.1816-1821.2005
dc.identifier.urihttp://hdl.handle.net/11603/13121
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Biological Sciences Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectanaerobic ammonia oxidation (anammox)en_US
dc.subjectChesapeake Bay basin areaen_US
dc.subjectInner Harbor of Baltimoreen_US
dc.titleAnaerobic Ammonia-Oxidizing Bacteria and Related Activity in Baltimore Inner Harbor Sedimenten_US
dc.typeTexten_US

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