Induced Spawning of F1 Wreckfish (Hapuku) ¯ Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone

Simple item page
dc.contributor.authorWylie, Matthew J.
dc.contributor.authorSetiawan, Alvin N.
dc.contributor.authorIrvine, Glen W.
dc.contributor.authorElizur, Abigail
dc.contributor.authorZohar, Yonathan
dc.contributor.authorSymonds, Jane E.
dc.contributor.authorLokman, P. Mark
dc.date.accessioned2019-10-09T15:40:56Z
dc.date.available2019-10-09T15:40:56Z
dc.date.issued2019-07-08
dc.description.abstractWild-caught hapuku ( ¯ Polyprion oxygeneios) spawn readily in captivity, but although first filial (F1) hapuku complete vitellogenesis, females fail to undergo oocyte maturation and spawn or produce poor quality eggs. This study investigated whether administration of a synthetic agonist of gonadotropin-releasing hormone (GnRHa) could improve F1 hapuku spawning and complete the life-cycle in captivity. Spawning trials were conducted over 2 years in 2013 and 2014, when F1 were aged five and six years. In 2013, females previously conditioned under a variable or constant temperature regime were implanted with GnRHa (100 µg/kg−1) or blank implants constructed of powdered cellulose and cholesterol. Spawning was erratic and egg quality very poor in all tanks. No F2 offspring were produced by communal spawning. In contrast, viable F2 larvae were produced by strip-spawning and in vitro fertilization after a series of GnRHa injections. In 2014, two additional trials were conducted: females received ethylene-vinyl acetate copolymer (EVAc) matrix implants containing GnRHa (100 µg/kg−1) or blank implants and in the second trial, two GnRHa doses (100 µg/kg−1 and 50 µg/kg−1) were tested. Eggs were first detected in all tanks 12–17 days post-implantation when females received 100 µg/kg−1 GnRHa implants, but not in the lower dose or control tanks. In summary, this study achieved induction of female spawning with GnRHa implants (target dose 100 µg/kg−1) and the successful production of F2 hapuku in captivity by strip-spawning.en_US
dc.description.sponsorshipThis research was supported by NIWA’s Research Aquaculture Program 3 (2013/2014; 2014/15 SCI) funding. A University of Otago Maori Doctoral Scholarship and the Nga Pae o te Maramatanga Doctoral Bridging Grant (17DBG02) to M.J.W. also supported this research.en_US
dc.format.extent16 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m2jgce-jk0f
dc.identifier.citationWylie, Matthew J.; Setiawan, Alvin N.; Irvine, Glen W.; Elizur, Abigail; Zohar, Yonathan; Symonds, Jane E.; Lokman, P. M. 2019. "Induced Spawning of F1 Wreckfish (Hāpuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone." Fishes 4, no. 3: 41.en_US
dc.identifier.urihttps://doi.org/10.3390/fishes4030041
dc.identifier.urihttp://hdl.handle.net/11603/15007
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution 4.0 International (CC BY 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectPolyprionen_US
dc.subjectspawning inductionen_US
dc.subjectoocyte maturationen_US
dc.subjectaquacultureen_US
dc.subjectovulationen_US
dc.subjectGnRHaen_US
dc.subjectgonadotropinen_US
dc.subjectwreckfishen_US
dc.subjectbroodstocken_US
dc.subjecthapukuen_US
dc.titleInduced Spawning of F1 Wreckfish (Hapuku) ¯ Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormoneen_US
dc.typeTexten_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Induced Spawning of F1 Wreckfish (Hāpuku) Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone.pdf
Size:
1.21 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.56 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UMBC Department of Marine Biotechnology
UMBC Faculty Collection