Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product

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dc.contributor.authorSchreier, Harold J.
dc.contributor.authorFisher, Susan H.
dc.contributor.authorSonenshein, Abraham L.
dc.date.accessioned2019-03-22T14:22:42Z
dc.date.available2019-03-22T14:22:42Z
dc.date.issued1985-05
dc.description.abstractExpression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than in the same strain under nitrogen-excess conditions. Mutations in the E. coli glnA, glnB, glnD, glnE, glnF, glnG, and glnL genes had no effect on the observed regulation. To test whether sequences within the B. subtilis DNA (3.2 kilobase pairs) were responsible for the observed regulation, a plasmid carrying a transcriptional fusion of the B. subtilis glnA promoter with E. coli lacZ was constructed. beta-Galactosidase levels coded for by this plasmid were found to be negatively regulated in trans by a plasmid carrying the entire B. subtilis glnA gene. Analysis of various deletion plasmids showed that the 1.4-kilobase-pair region encoding glutamine synthetase was necessary for the observed regulation of beta-galactosidase. Plasmids coding for 67% or more of the glutamine synthetase polypeptide gave at least partial repression, but a plasmid carrying 30% of the structural gene, as well as a plasmid carrying a deletion internal to glnA, gave no repression. DNA downstream from glnA (to within 130 base pairs of the end of the gene) was not required for the observed regulation. These results suggest that the glnA gene of B. subtilis is autoregulated, supporting the model for glnA control proposed by Dean et al. [Dean, D. R., Hoch, J. A. & Aronson, A. I. (1977) J. Bacteriol. 131, 981-987].en_US
dc.description.sponsorshipThis work was supported by Research Grant GM 19168 to A.L.S. and Postdoctoral Fellowship F32GM 09430-01 to H.J.S., both from the National Institutes of Health.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/2860669en_US
dc.format.extent5 pagesen_US
dc.genrejournal articlesen_US
dc.identifierdoi:10.13016/m21x94-ob8r
dc.identifier.citationH J Schreier, S H Fisher, and A L Sonenshein, Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product, Proc Natl Acad Sci U S A. 1985 May;82(10):3375-9, https://www.ncbi.nlm.nih.gov/pubmed/2860669en_US
dc.identifier.urihttp://hdl.handle.net/11603/13122
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Biological Sciences Department
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.subjectglnA geneen_US
dc.subjectBacillus subtilisen_US
dc.subjectEscherichia colien_US
dc.titleRegulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene producten_US
dc.typeTexten_US

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