V.K. Gupta ,C.D. Eggleton, A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy, Colloids Surf B Biointerfaces. 2012 June 15; 95: 50–56. doi:10.1016/j.colsurfb.2012.02.010
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Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 10³ – 10⁶ pN/s.
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