A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy

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nihms360468.pdf (1.63 MB)

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348403/

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10.1016/j.colsurfb.2012.02.010
 http://hdl.handle.net/11603/11624

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UMBC Mechanical Engineering Department
UMBC Faculty Collection

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2012-06-15

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journal article pre-print
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Citation of Original Publication

V.K. Gupta ,C.D. Eggleton, A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy, Colloids Surf B Biointerfaces. 2012 June 15; 95: 50–56. doi:10.1016/j.colsurfb.2012.02.010

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Subjects

kinetic off-rates
multiple bond force
spectroscopy
Monte-Carlo simulations
UMBC High Performance Computing Facility (HPCF)

Abstract

Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 10³ – 10⁶ pN/s.