A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy

Eggleton, C.D.; Gupta, V.K.

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Author/Creator

Eggleton, C.D.

Gupta, V.K.

Date

2012-06-15

Type of Work

23 pages
Text
journal article pre-print

Citation of Original Publication

V.K. Gupta ,C.D. Eggleton, A Theoretical Method to determine unstressed off-rate from multiple bond force spectroscopy, Colloids Surf B Biointerfaces. 2012 June 15; 95: 50–56. doi:10.1016/j.colsurfb.2012.02.010

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Subjects

kinetic off-rates
multiple bond force
spectroscopy
Monte-Carlo simulations
UMBC High Performance Computing Facility (HPCF)

Abstract

Using dynamic force spectroscopy to measure the kinetic off-rates of intermolecular bonds currently requires the isolation of single molecules. This requirement arises in part because no tractable analytic method for determining kinetic off-rates from the rupture of a large number of bonds under dynamic forces is currently available. We introduce a novel method for determining the unstressed off-rate from dynamic force spectroscopy experiments involving a large number of bonds. Using both the Bell and Dembo models we show that the unstressed off-rate calculated using the proposed method is in good agreement with the prescribed unstressed off-rate used in Monte-Carlo simulations of multiple bond dynamic force spectroscopy experiments given initial number of bonds (50–500) and loading rate 10³ – 10⁶ pN/s.

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This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.

Except where otherwise noted, this item's license is described as This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.