Browsing UMBC Department of Marine Biotechnology by Title
Now showing items 28-47 of 59
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Identification and characterization of the pckA gene from Staphylococcus aureus
(American Society for Microbiology, 1996-06)The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA ... -
Induced Spawning of F1 Wreckfish (Hapuku) ¯ Polyprion oxygeneios Using a Synthetic Agonist of Gonadotropin-Releasing Hormone
(MDPI, 2019-07-08)Wild-caught hapuku ( ¯ Polyprion oxygeneios) spawn readily in captivity, but although first filial (F1) hapuku complete vitellogenesis, females fail to undergo oocyte maturation and spawn or produce poor quality eggs. ... -
Intensive oyster aquaculture can reduce disease impacts on sympatric wild oysters
(Inter-Research, 2018-12-13)Risks associated with disease spread from fish and shellfish farming have plagued the growth and public perception of aquaculture worldwide. However, by processing nutrients and organic material from the water column, ... -
Interaction of the Bacillus subtilis glnRA repressor with operator and promoter sequences in vivo
(American Society for Microbiology (ASM), 1992-02)In vivo dimethyl sulfate footprinting of the Bacillus subtilis ginRA regulatory region under repressing and derepressing conditions demonstrated that the GlnR protein, encoded by glnR, interacts with two sites situated within ... -
Intranasal vaccination of hamsters with a Newcastle disease virus vector expressing the S1 subunit protects animals against SARS-CoV-2 disease
(Nature, 2022-06-20)The coronavirus disease-19 (COVID-19) pandemic has already claimed millions of lives and remains one of the major catastrophes in the recorded history. While mitigation and control strategies provide short term solutions, ... -
Investigation into the fungal diversity within different regions of the gastrointestinal tract of Panaque nigrolineatus, a wood-eating fish
(AIMS Press, 2017-09-04)The Amazonian catfish, Panaque nigrolineatus have several physiological adaptions enabling the scraping and consumption of wood (xylivory), facilitating a detritivorous dietary strategy. Composed of lignocellulose, wood ... -
Isolation, Identification, and Genomic Analysis of a Novel Reovirus from Healthy Grass Carp and Its Dynamic Proliferation In Vitro and In Vivo
(MDPI, 2021-04-16)A new grass carp reovirus (GCRV), healthy grass carp reovirus (HGCRV), was isolated from grass carp in 2019. Its complete genome sequence was determined and contained 11 dsRNAs with a total size of 23,688 bp and 57.2 mol% ... -
Macrobrachium rosenbergii nodavirus ( Mr NV)-CP-RNA-2 DNA vaccine confers protective immunity in giant freshwater prawn Macrobrachium rosenbergii against Mr NV infection
(Elsevier B.V., 2019-03)Macrobrachium rosenbergii Nodavirus (MrNV) causes white tail disease (WTD) in Giant freshwater prawn Macrobrachium rosenbergii which leads to immense economic losses in hatcheries and farms. In the present study, we cloned ... -
The NanDeSyn database for Nannochloropsis systems and synthetic biology
(Wiley, 2020-10-25)Nannochloropsis species, unicellular industrial oleaginous microalgae, are model organisms for microalgal systems and synthetic biology. To facilitate community‐based annotation and mining of the rapidly accumulating ... -
Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens
(MDPI, 2021-10-02)Newcastle disease virus (NDV) strain R2B, with an altered fusion protein cleavage site, was used as a viral vector to deliver the immunogenic genes VP2 and VP1 of chicken infectious anaemia virus (CIAV) to generate a ... -
Nitrogenase diversity and activity in the gastrointestinal tract of the wood-eating catfish Panaque nigrolineatus
(International Society for Microbial Ecology, 2015-04-24)The Amazonian catfish, Panaque nigrolineatus, consume large amounts of wood in their diets. The nitrogen-fixing community within the gastrointestinal (GI) tract of these catfish was found to include nifH phylotypes that ... -
The Nucleoprotein and Phosphoprotein Are Major Determinants of the Virulence of Viral Hemorrhagic Septicemia Virus in Rainbow Trout
(American Society for Microbiology, 2019-09-15)Viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus, infects several marine and freshwater fish species. There are many strains of VHSV that affect different fish, but some strains of one genetic subgroup have ... -
Oral vaccination of Macrobrachium rosenbergii with baculovirus-expressed M. rosenbergii nodavirus (MrNV) capsid protein induces protective immunity against MrNV challenge
(Elsevier B.V, 2019-03)White Tail Disease (WTD) is one of the important viral diseases of fresh water giant prawn Macrobrachium rosenbergii, which is caused by Macrobrachium rosenbergii nodavirus (MrNV). In the present study, the capsid protein ... -
Pacific oysters are a sink and a potential source of the eelgrass pathogen, Labyrinthula zosterae
(Inter-Research, 2022-12-15)Oyster aquaculture and seagrasses often co-occur and are each vital to the ecological and economic value of coastal ecosystems. Global declines in seagrasses, including Zostera mari - na, have recently been observed in ... -
Phylogenetic Analysis of Microbial Communities in Different Regions of the Gastrointestinal Tract in Panaque nigrolineatus, a Wood-Eating Fish
(PLOS, 2012-10-25)The Neotropical detritivorous catfish Panaque nigrolineatus imbibes large quantities of wood as part of its diet. Due to the interest in cellulose, hemi-cellulose and lignin degradation pathways, this organism provides an ... -
Production of reproductively sterile fish by a non-transgenic gene silencing technology
(Nature, 2015-10-29)We developed a novel bath-immersion technology to produce large numbers of infertile fish. As seafood consumption shifts from fishery harvests towards artificially propagated species, optimization of aquaculture practices ... -
Purification and properties of glutamate synthase from Bacillus licheniformis
(American Society for Microbiology, 1984-11)Glutamate synthase [L-glutamate:NADP+ oxidoreductase (transaminating); EC 1.4.1.13] (GItS) was purified to homogeneity from BaciUus licheniformis A5. The native enzyme had a molecular weight of approximately 220,000 and ... -
Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens
(MDPI, 2019-09-10)Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian ... -
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product
(American Society for Microbiology, 1985-05)Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than in the same strain under ... -
Regulation of nitrogen catabolic enzymes in Bacillus spp
(American Society for Microbiology, 1982-08)The levels of the inducible nitrogen catabolic enzymes arginase (L-arginine amidinohydrolase, EC 3.5.3.1) and alanine dehydrogenase (L-alanine:NAD+ oxidoreductase [deaminating], EC 1.4.1.1) from Bacillus licheniformis and ...