Browsing UMBC Department of Marine Biotechnology by Issue Date
Now showing items 1-20 of 30
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Regulation of nitrogen catabolic enzymes in Bacillus spp
(American Society for Microbiology, 1982-08)The levels of the inducible nitrogen catabolic enzymes arginase (L-arginine amidinohydrolase, EC 3.5.3.1) and alanine dehydrogenase (L-alanine:NAD+ oxidoreductase [deaminating], EC 1.4.1.1) from Bacillus licheniformis and ... -
Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis
(American Society for Microbiology, 1984-08)The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive ,β-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. -
Purification and properties of glutamate synthase from Bacillus licheniformis
(American Society for Microbiology, 1984-11)Glutamate synthase [L-glutamate:NADP+ oxidoreductase (transaminating); EC 1.4.1.13] (GItS) was purified to homogeneity from BaciUus licheniformis A5. The native enzyme had a molecular weight of approximately 220,000 and ... -
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product
(American Society for Microbiology, 1985-05)Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than in the same strain under ... -
Altered regulation of the glnA gene in glutamine synthetase mutants of Bacillus subtilis
(American Society for Microbiology, 1986-07-01)Expression of beta-galactosidase by Bacillus subtilis strains carrying transcriptional fusions of the glnA promoter region to the Escherichia coli lacZ gene was found to be regulated by the nitrogen source in glnA+ strains. ... -
Interaction of the Bacillus subtilis glnRA repressor with operator and promoter sequences in vivo
(American Society for Microbiology (ASM), 1992-02)In vivo dimethyl sulfate footprinting of the Bacillus subtilis ginRA regulatory region under repressing and derepressing conditions demonstrated that the GlnR protein, encoded by glnR, interacts with two sites situated within ... -
Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase
(American Society for Microbiology, 1993-02)A Bacillus subfilis mutant that produced glutamine synthetase (GS) with altered sensitivity to DL-methionine sulfoximine was isolated. The mutation, designated glnA33, was due to a T- A-to-C- G transition, changing valine ... -
Identification and characterization of the pckA gene from Staphylococcus aureus
(American Society for Microbiology, 1996-06)The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA ... -
β-Glutamate as a Substrate for Glutamine Synthetase
(American Society for Microbiology, 2001-10)The conversion of β-glutamate to β-glutamine by archaeal and bacterial glutamine synthetase (GS) enzymes has been examined. The GS from Methanohalophilus portucalensis (which was partially purified) is capable of catalyzing ... -
Anaerobic Ammonia-Oxidizing Bacteria and Related Activity in Baltimore Inner Harbor Sediment
(American Society for Microbiology, 2005-04)The discovery of bacteria capable of anaerobic ammonia oxidation (anammox) has generated interest in understanding the activity, diversity, and distribution of these bacteria in the environment. In this study anammox ... -
Stimulating Denitrification in a Marine Recirculating Aquaculture System Biofilter Using Granular Starch as a Carbon Source
(Internal Journal of Recirculating Aquaculture, 2008)Maintaining superior water quality in intensive recirculating aquaculture systems (RAS) by controlling levels of inorganic nitrogenous waste—ammonia, nitrate and nitrite—derived from uneaten food and fecal excretion is ... -
Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant
(American Society for Microbiology, 2012-10)A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic ... -
Phylogenetic Analysis of Microbial Communities in Different Regions of the Gastrointestinal Tract in Panaque nigrolineatus, a Wood-Eating Fish
(PLOS, 2012-10-25)The Neotropical detritivorous catfish Panaque nigrolineatus imbibes large quantities of wood as part of its diet. Due to the interest in cellulose, hemi-cellulose and lignin degradation pathways, this organism provides an ... -
Examination of a Culturable Microbial Population from the Gastrointestinal Tract of the Wood-Eating Loricariid Catfish Panaque nigrolineatus
(MDPI, 2013-08-19)Fish play a critical role in nutrient cycling and organic matter flow in aquatic environments. However, little is known about the microbial diversity within the gastrointestinal tracts that may be essential in these ... -
Effect of sulfide on growth of marine bacteria
(Springer Nature Switzerland AG., 2014-03-09)Severe hypoxia leads to excess production of hydrogen sulfide in marine environments. In this study, we examined the effect of sulfide on growth of four facultative anaerobic marine bacteria in minimal media under anaerobic ... -
Draft Genome Sequence of the Shellfish Bacterial Pathogen Vibrio sp. Strain B183
(American Society for Microbiology, 2014-09-18)We report the draft genome sequence of Vibrio sp. strain B183, a Gram-negative marine bacterium isolated from shellfish that causes mortality in larval mariculture. The availability of this genome sequence will facilitate ... -
Draft Genome Sequence of the Oyster Larval Probiotic Bacterium Vibrio sp. Strain OY15
(American Society for Microbiology, 2014-10-02)We report the draft genome sequence of Vibrio sp. strain OY15, a Gram-negative marine bacterium isolated from an oyster (Crassostrea virginica) digestive tract and shown to possess probiotic activity. The availability of ... -
Nitrogenase diversity and activity in the gastrointestinal tract of the wood-eating catfish Panaque nigrolineatus
(International Society for Microbial Ecology, 2015-04-24)The Amazonian catfish, Panaque nigrolineatus, consume large amounts of wood in their diets. The nitrogen-fixing community within the gastrointestinal (GI) tract of these catfish was found to include nifH phylotypes that ... -
Production of reproductively sterile fish by a non-transgenic gene silencing technology
(Nature, 2015-10-29)We developed a novel bath-immersion technology to produce large numbers of infertile fish. As seafood consumption shifts from fishery harvests towards artificially propagated species, optimization of aquaculture practices ... -
Draft Genome Sequence of Vibrio (Listonella) anguillarum ATCC 14181
(American Society for Microbiology, 2016-10-20)We report the draft genome sequence of Vibrio anguillarum ATCC 14181, a Gram-negative, hemolytic, O2 serotype marine bacterium that causes mortality in mariculture species. The availability of this genome sequence will add ...