Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus
No Thumbnail Available
Links to Files
Permanent Link
Author/Creator
Author/Creator ORCID
Date
2017-03-03
Type of Work
Department
Program
Citation of Original Publication
Islam N, Hossain KG, Ross JM, Marten MR, Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus, Adv Biotechnol Microbiol. 2017;2(3). pii: 555589. doi: 10.19080/ARGH.2017.02.555589
Rights
This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
Subjects
Abstract
Background:
S. aureus biofilm serves a major role in pathogenesis. Two of the major components of bacterial biofilm are Polysaccharides intercellular adhesions (PIA) and surface proteins. It is not known how PIA and surface proteins expressions are affected in presence of blood serum. Analyses of surface proteins expressions will provide more effective biomarker discovery that might lead to development of antimicrobial therapeutics to meet the challenges of biofilm-related infections.
Method:
Secondary cultures of S. aureus Philips, a biofilm-forming bacterium, were generated by inoculating 1 ml of overnight culture into 50 ml of TSB. Bacteria were cultured at several concentrations of blood serum and found that 12.5% supplemented blood serum provide s similar growth curve as normal TSB (100%). One and 2 D SASPAGE were used to separate proteins and the differentially expressed proteins were identified by nano-LC/MS.
Results:
Polysaccharide intercellular adhesions production was significantly increased due to the addition of blood serum in the media. We also identified two serum proteins, apolipoprotein and globulin (Fc and Fab), that remained attached with the membrane fraction of bacterial proteins.
Conclusion:
These results have strongly demonstrated that blood serum influences the exopolysaccharide expression in S. aureus.