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dc.contributor.authorCitarasu, Thavasimuthu
dc.contributor.authorLelin, Chinnadurai
dc.contributor.authorBabu, Mariavincent Michael
dc.contributor.authorAnand, Setty Balakrishnan
dc.contributor.authorNathan, Abel Arul
dc.contributor.authorVakharia, Vikram N.
dc.date.accessioned2019-02-14T16:00:28Z
dc.date.available2019-02-14T16:00:28Z
dc.date.issued2019-03
dc.description.abstractWhite Tail Disease (WTD) is one of the important viral diseases of fresh water giant prawn Macrobrachium rosenbergii, which is caused by Macrobrachium rosenbergii nodavirus (MrNV). In the present study, the capsid protein gene of MrNV containing a His-tag was cloned into a baculovirus vector pVL1393 and expressed the recombinant MrNV protein in insect cells, using a baculovirus expression system. A band corresponding to the MrNV protein of 43 kDa was characterized after fractionating the proteins of baculovirus-infected cell lysates by SDS-polyacrylamide gel, and immunostaining with His-tag monoclonal antibody. Furthermore, purified MrNV capsid protein assembled into virus-like particles (VLPs) of ∼30 nm in diameter, when examined by transmission electron microscopy (TEM). To vaccinate the larvae by oral route, the recombinant MrNV (r-MrNV) protein was coated with artificial prawn feed and fed to M. rosenbergii larvae (90 ± 10 mg) for 60 days. After 30 and 60 days of vaccine treatment, group of prawns were challenged with virulent MrNV orally. Samples were collected at different time intervals to evaluate the survival of larvae and to analyze the presence of MrNV by double-step PCR and expression of immune/ toll-like receptor (TLR) genes. Non-vaccinated group of M. rosenbergii larvae succumbed to death and had 90% mortality, whereas the r-MrNV protein treated groups exhibited 65 and 80% survival (P  ≤  0.001) for 30 and 60 days post-vaccination (dpv), respectively. Double-step PCR diagnosis revealed that there was 100% positive signals observed in non-vaccinated prawn group, whereas the infection was reduced significantly (P < 0.001) to 32 and 17% respectively in 30 and 60 dpv. Among the four different immune/ TLR genes such as antimicrobial peptide (Mramp), lysozyme (MrLY), proPhenol Oxidase (MrPPO) and Toll-Like Receptor (MrToll) expression screening, Mramp was successfully expressed in the MrNV subunit protein vaccinated prawns, whereas the non-vaccinated prawn had no immune/TLR gene expression. Taken together, our results demonstrate that oral vaccination of M. rosenbergii larvae with baculovirus-expressed MrNV capsid protein confer up to 78% protection against MrNV infection.en_US
dc.description.sponsorshipDr. T. Citarasu, gratefully acknowledges the University Grants Commission (UGC), Government of India, New Delhi for the financial support, in the form of Raman Postdoctoral Fellowship (No.F. 5-37/ 2014 (IC) dtd 26-08-2014) and Institute of Marine and Environmental Technology (IMET), University of Maryland, Baltimore County, Columbus Center, Baltimore, Maryland (MD), United States of America for the host lab support.en_US
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S105046481830809Xen_US
dc.format.extent7 pagesen_US
dc.genrejournal articles postprintsen_US
dc.identifierdoi:10.13016/m2ki3p-ipkh
dc.identifier.citationThavasimuthu Citarasu , Chinnadurai Lelin , Mariavincent Michael Babu , Setty Balakrishnan Anand , Abel Arul Nathan , Vikram N. Vakharia , Oral vaccination of Macrobrachium rosenbergii with baculovirus-expressed M. rosenbergii nodavirus (MrNV) capsid protein induces protective immunity against MrNV challenge, Fish & Shellfish Immunology , Volume 86, March 2019, Pages 1123-1129, https://doi.org/10.1016/j.fsi.2018.12.010en_US
dc.identifier.urihttps://doi.org/10.1016/j.fsi.2018.12.010
dc.identifier.urihttp://hdl.handle.net/11603/12788
dc.language.isoen_USen_US
dc.publisherElsevier B.Ven_US
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Department of Marine Biotechnology
dc.relation.ispartofUMBC Faculty Collection
dc.rightsThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.rights.uriAccess to this item will begin on March 31. 2020*
dc.subjectbaculovirus expressionen_US
dc.subjectmacrobrachium rosenbergii nodavirus (MrNV)en_US
dc.subjectrecombinant subunit vaccinationen_US
dc.subjectimmune gene expressionen_US
dc.titleOral vaccination of Macrobrachium rosenbergii with baculovirus-expressed M. rosenbergii nodavirus (MrNV) capsid protein induces protective immunity against MrNV challengeen_US
dc.typeTexten_US


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