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    DEVELOPMENT OF A REAL-TIME PCR ASSAY FOR MOUSE FUR MITES AND EVALUATION OF PILOT STUDY

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    thesis (922.7Kb)
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    http://hdl.handle.net/11603/13129
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    • Hood College Biomedical and Environmental
    • Hood College Student Works
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    Author/Creator
    Poore, Allison
    Date
    2019-03-22
    Type of Work
    67 pages
    Text
    Thesis
    Department
    Biomedial Science
    Program
    Hood College Graduate School Biomedical Science Program
    Subjects
    Biology, Molecular (0307)
    Abstract
    Mouse fur mites Myobia musculi and Myocoptes musculinus are common parasites of mice and present a unique challenge for exclusion from laboratory animal facilities. In this study, a real-time PCR test was developed to detect both mouse fur mites using distinct dye in a single reaction well. Confirmatory endpoint PCR tests were also designed, to confirm equivocal results. The assays were then tested during a pilot study, where known positive animals were used to evaluate the accuracy of environmental targets for screening. In all cages were mice were positive for the fur mite real-time PCR, environmental targets were also positive within 10 days of placement in a new, clean cage. This study shows that the real-time PCR assay and environmental targets are an effective combination for screening of mouse fur mites in laboratory animal facilities.


    Beneficial-Hodson Library
    Hood College
    401 Rosemont Avenue
    Frederick, MD 21701
    www.hood.edu


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.

     

     

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    Beneficial-Hodson Library
    Hood College
    401 Rosemont Avenue
    Frederick, MD 21701
    www.hood.edu


    If you wish to submit a copyright complaint or withdrawal request, please email mdsoar-help@umd.edu.