Search
Now showing items 1-10 of 15
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product
(American Society for Microbiology, 1985-05)
Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than in the same strain under ...
Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli
(Oxford University Press, 1991-05-25)
We have analyzed the processing of mRNA from the lac operon in an Escherichia coli strain carrying the lac on a multicopy plasmid. Messenger RNA was analyzed by hybridization and nuclease protection of pulse-labeled RNA ...
Secondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operon
(Oxford University Press, 1988-09-26)
Genetic analysis of the autogenous control of the S10 ribosomal protein operon of Escherichia coli has suggested that the secondary or tertiary structure of the leader transcript is important for this regulation. We have ...
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli
(Oxford University Press, 1985-06-11)
In Escherichia coli some 19 transcription units encoding the 52 ribosomal proteins are scattered throughout the genome. One of the units, the alpha operon, encodes genes for the ribosomal proteins S13, S11, S4 and L17 as ...
Phylogenetic Analysis of L4-Mediated Autogenous Control of the S10 Ribosomal Protein Operon
(American Society for Microbiology, 1999-07-25)
We investigated the regulation of the S10 ribosomal protein (r-protein) operon among members of the
gamma subdivision of the proteobacteria, which includes Escherichia coli. In E. coli, this 11-gene operon is
autogenously ...
Precursor for elongation factor Tu from Escherichia coli
(American Society for Microbiology, 1986-02)
The tufA gene, one of two genes in Escherichia coli encoding elongation factor Tu (EF-Tu), was cloned into a ColE1-derived plasmid downstream of the lac promoter-operator. In cells carrying this plasmid, the synthesis of ...
Translational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia coli
(American Society for Microbiology, 1989-05)
We have examined the translational coupling between the first two genes in the S10 ribosomal protein operon. We isolated mutations blocking the translation of the first gene of the operon, coding for S10, and monitored ...
Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli
(American Society for Microbiology, 1990-01)
The regulation of the S10 ribosomal protein operon of Escherichia coli was studied by using a lambda prophage containing the beginning of the S10 operon (including the promoter, leader, and first one and one-half structural ...
Transcriptional control of the S10 ribosomal protein operon of Escherichia coli after a shift to higher temperature
(American Society for Microbiology, 1985-07)
In the 5 to 10 min immediately following a shift from 30 to 42 degrees C, the differential synthesis rates of ribosomal proteins encoded by the 11-gene S10 operon are transiently decreased. This effect results largely from ...
Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation
(National Academy of Sciences, 1987-09)
The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting ...