Browsing UMBC Faculty Collection by Issue Date
Now showing items 1-20 of 1974
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Specialized transducing phages for ribosomal protein genes of Escherichia coli
(National Academy of Sciences, 1975-01-01)Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein ... -
Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits
(National Academy of Sciences, 1975-07-01)This work was supported in part by the College of Agriculture and Life Sciences, University of Wisconsin, and by grants from the National Science Foundation (GB31086) and the National Institute of General Medical Sciences ... -
Mapping of ribosomal protein genes by in vitro protein synthesis using DNA fragments of lambda fus3 transducing phage DNA as templates
(1977-10-25)hfus3 (or hfus2) transducing phage stimulates the synthesis of 27 ribosomal proteins, elongation factors EF-Tu and EF-G, and RNA polymerase subunit (Y in ultraviolet-irradiated Escherichia roli cells (see a preceding ... -
Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli
(American Society for Microbiology, 1977-12)We have screened the strains with ColE1 hybrid plasmids constructed by Clarke and Carbon (Cell 9:91-99, 1976) for the presence of ribosomal ribonucleic acid (rRNA) genes on the plasmids and identified 16 strains whose ... -
Operon-specific regulation of ribosomal protein synthesis in Escherichia coli
(National Academy of Sciences, 1979-12)We have cloned a DNA fragment harboring the genes for ribosomal proteins L2, L4, and L23 on a plasmid vector that contains a lac operator and promoter. The cloned ribosomal protein genes are now under the control of lacOP. ... -
High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli
(American Society for Microbiology (ASM), 1981-01)We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% ... -
Oversynthesis of elongation factors G and Tu in Escherichia coli
(American Society for Microbiology (ASM), 1982-02)We induced the oversynthesis of elongation factors Tu and G by using multicopy plasmids carrying the structural genes for these proteins under the control of the lac operator-promoter. We found no evidence that accumulation ... -
The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.
(Oxford University Press, 1984-02-24)We have determined the nucleotide sequence of the Escherichia coli fus gene, which codes for elongation factor G. The protein product of the sequenced gene contains 703 amino acids, with a predicted molecular weight of ... -
Role of attenuation in growth rate-dependent regulation of the S10 r-protein operon of E. coli
(The European Molecular Biology Organization, 1984-07)We have investigated the transcription of the 11 gene S10 ribosomal protein operon of Escherichia coli under various growth conditions. The differential synthesis rate of structural gene message increases 2- to 2.5-fold ... -
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli
(Oxford University Press, 1985-06-11)In Escherichia coli some 19 transcription units encoding the 52 ribosomal proteins are scattered throughout the genome. One of the units, the alpha operon, encodes genes for the ribosomal proteins S13, S11, S4 and L17 as ... -
Transcriptional control of the S10 ribosomal protein operon of Escherichia coli after a shift to higher temperature
(American Society for Microbiology, 1985-07)In the 5 to 10 min immediately following a shift from 30 to 42 degrees C, the differential synthesis rates of ribosomal proteins encoded by the 11-gene S10 operon are transiently decreased. This effect results largely from ... -
Precursor for elongation factor Tu from Escherichia coli
(American Society for Microbiology, 1986-02)The tufA gene, one of two genes in Escherichia coli encoding elongation factor Tu (EF-Tu), was cloned into a ColE1-derived plasmid downstream of the lac promoter-operator. In cells carrying this plasmid, the synthesis of ... -
Transcription of ribosomal genes during a nutritional shift-up of Escherichia coli
(American Society for Microbiology, 1986-09)We measured the differential transcription rates of individual ribosomal operons after a nutritional shift-up. All operons showed a transient increase in transcription. However, the response of the S10 ribosomal protein ... -
Spectral sensitivity of the compound eyes in the purple land crab, Gecarcinus lateralis
(1987-08-17)The spectral sensitivities(S(λ)) of dark-adapted compound eyes of the purple land crab Gecarcinus lateralis posses a broad maximum in the blue-green, 420-530 nm,when measured by electroretinographic (ERG) techniques. ... -
Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation
(National Academy of Sciences, 1987-09)The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting ... -
Ocular Tracking of Rapidly Moving Visual Targets by Stomatopod Crustaceans
(Company of Biologists, 1988)1. Ocular tracking in two species of stomatopod crustaceans, Squilla empusa (Say) and Gonodactylus oerstedii (Hansen), was studied by presenting animals with small targets moving to their right and left. 2. Squilla ... -
Secondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operon
(Oxford University Press, 1988-09-26)Genetic analysis of the autogenous control of the S10 ribosomal protein operon of Escherichia coli has suggested that the secondary or tertiary structure of the leader transcript is important for this regulation. We have ... -
Translational coupling of the two proximal genes in the S10 ribosomal protein operon of Escherichia coli
(American Society for Microbiology, 1989-05)We have examined the translational coupling between the first two genes in the S10 ribosomal protein operon. We isolated mutations blocking the translation of the first gene of the operon, coding for S10, and monitored ... -
Spectral Sensitivity of Vision in the Mantis Shrimp, Gonodactylus oerstedii, Determined Using Noninvasive Optical Techniques
(The Biological bulletin, 1989-06-01)Compound eyes of stomatopod crustaceans have many unique design features. Recently developed intracellular optical physiology techniques permit the measurement of spectral sensitivity functions in intact eyes of these ... -
Autogenous control is not sufficient to ensure steady-state growth rate-dependent regulation of the S10 ribosomal protein operon of Escherichia coli
(American Society for Microbiology, 1990-01)The regulation of the S10 ribosomal protein operon of Escherichia coli was studied by using a lambda prophage containing the beginning of the S10 operon (including the promoter, leader, and first one and one-half structural ...