Using Redox Sensitive Dye for Expedited Antibiotic Minimum Inhibitory Concentration Analysis of Tier 1 Bacterial BSAT
Loading...
Links to Files
Permanent Link
Author/Creator
Author/Creator ORCID
Date
Department
Hood College Biology Department
Program
Hood College Biomedical and Environmental
Citation of Original Publication
Rights
Attribution-NonCommercial-NoDerivs 3.0 United States
Abstract
Here, we present an optimized colorimetric broth microdilution assay for Bacillus
anthracis and Yersinia pestis that includes the redox sensitive dye resazurin added to the
media. We found the assay results were easier to interpret and gave results significantly
faster than the gold standard broth microdilution assay outlined in the CLSI guidelines.
We obtained results in roughly half the time; 5h instead of 24h for B. anthracis and 28h
instead of 48h for Y. pestis. Among 32 strains of B. anthracis tested with three different
antibiotics and 25 strains of Y. pestis tested with 4 antibiotics, the assay provided
categorical agreement of 100% and 99.7%, respectively. Essential agreement was the same
for both bacteria, 99.3%. We also eliminated MIC determination subjectivity by measuring
the color shift using a color detection app via smartphone and creating an equation to
interpret the results.