Human cytokine response to herpes simplex virus type 2 infection in keratinocytes: the antiherpetic contribution
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Author/Creator ORCID
Date
2015-09-08
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Department
Towson University. Department of Biological Sciences
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Citation of Original Publication
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There are no restrictions on access to this document. An internet release form signed by the author to display this document online is on file with Towson University Special Collections and Archives.
There are no restrictions on access to this document. An internet release form signed by the author to display this document online is on file with Towson University Special Collections and Archives.
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Abstract
Herpes simplex virus type 2 (HSV-2) is a common genital ulcer disease with no cure. While therapeutics are available, the immune response to therapeutics and HSV-2 infection are not fully understood. In this study, cytokine expression with and without HSV-2 infection and anti-HSV-2 drugs was measured in primary keratinocytes. At the protein level, there was a marked increase in sICAM-1, IFN-y, and Serpin E1 12 hours after HSV-2 infection; an increase in SerpinE1 and sICAM-1 was seen 6 hours post-infection with HSV-2 and acyclovir treatment; and a slight decrease in Serpin E1 was seen 6 hours post-infection with cidofovir treatment. Serpin E1 mRNA was decreased in all aforementioned groups. These results confirm that keratinocytes play a role in the initial immune response to HSV-2, that some HSV-2-induced cytokine induction occurs by 12 hours post-infection, and that some cellular or HSV-2 regulation of Serpin E1 mRNA may occur in the presence of ACV.