Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus
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2021-03-19
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Kloetzel, John A.; Aubusson‐Fleury, Anne; Butler, Maurice D.; Banerjee, Deben; Mozzicafreddo, Matteo; Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus; Eukaryotic Microbiology (2021); https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850
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This is the peer reviewed version of the following article: Kloetzel, John A.; Aubusson‐Fleury, Anne; Butler, Maurice D.; Banerjee, Deben; Mozzicafreddo, Matteo; Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus; Eukaryotic Microbiology (2021); https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850 ,which has been published in final form at https://doi.org/10.1111/jeu.12850. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Access to this item will begin on 2022-03-19
This is the peer reviewed version of the following article: Kloetzel, John A.; Aubusson‐Fleury, Anne; Butler, Maurice D.; Banerjee, Deben; Mozzicafreddo, Matteo; Sequence and Properties of Cagein, a Coiled‐Coil Scaffold Protein Linking Basal Bodies in the Polykinetids of the Ciliate Euplotes aediculatus; Eukaryotic Microbiology (2021); https://onlinelibrary.wiley.com/doi/10.1111/jeu.12850 ,which has been published in final form at https://doi.org/10.1111/jeu.12850. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Access to this item will begin on 2022-03-19
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Abstract
In the hypotrich ciliate Euplotes, many individual basal bodies are grouped together in tightly packed clusters, forming ventral polykinetids. These groups of basal bodies (which produce compound ciliary organelles such as cirri and oral membranelles) are cross‐linked into ordered arrays by scaffold structures known as ‘basal body cages’. The major protein comprising Euplotes cages has been previously identified and termed ‘cagein’. Screening a E. aediculatus cDNA expression library with anti‐cagein antisera identified a DNA insert containing most of a putative cagein gene; standard PCR techniques were used to complete the sequence. Probes designed from this gene identified a macronuclear ‘nano‐chromosome’ of ca. 1.5 kb in Southern blots against whole‐cell DNA. The protein derived from this sequence (463 residues) is predicted to be hydrophilic and highly charged; however, the native cage structures are highly resistant to salt/detergent extraction. This insolubility could be explained by the coiled‐coil regions predicted to extend over much of the length of the derived cagein polypeptide. One frameshift sequence is found within the gene, as well as a short intron. BLAST searches find many ciliates with evident homologues to cagein within their derived genomic sequences.