Bioengineering Novel Floating Nanoparticles for Protein and Drug Delivery
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Type of Work8 pages
Citation of Original PublicationDasSarma, P., Karan, R., Kim, J.-M., Pecher, W., & DasSarma, S. (January 01, 2016). Bioengineering Novel Floating Nanoparticles for Protein and Drug Delivery. Materials Today: Proceedings, 3, 2, 206-210.
Gas vesicle nanoparticles (GVNPs) are hollow protein nanoparticles produced by Halobacterium sp. NRC-1 which are being engineered for protein delivery. To advance the bioengineering potential of GVNPs, a strain of NRC-1 deleted for the gvpC gene (ΔgvpC) was constructed and a synthetic gene coding for Gaussia princeps luciferase was fused to an abbreviated gvpC gene on an expression plasmid. When introduced into theΔgvpC strain, an active GvpC-luciferase fusion protein bound to GVNPs resulted. These results represent both a technical improvement in the GVNP display system and its expansion for the display of active enzymes.