High-throughput isolation of oyster DNA facilitates diagnosis of "Dermo"
Loading...
Permanent Link
Collections
Author/Creator
Author/Creator ORCID
Date
2000
Type of Work
Department
Program
Citation of Original Publication
JAF Robledo, WT Pecher, GR Vasta. (2000). High-throughput isolation of oyster DNA facilitates diagnosis of "Dermo". Qiagen News 5, 15-17
Rights
Subjects
Abstract
The parasite Perkinsus marinus, the causative agent of ”Dermo” disease, has been linked with mass mortalities of the eastern oyster Crassostrea virginica along the Atlantic and Gulf Coasts of the United States. Here we describe a method for efficient high-throughput DNA isolation from oysters using the QIAGEN® DNeasy 96 Tissue Kit, and a sensitive, species-specific, PCR-based method for the diagnosis of P. marinus.