Browsing UMBC Chemical, Biochemical & Environmental Engineering Department by Issue Date
Now showing items 1-20 of 75
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Rheological Characterization and Biological and Mass Transfer Kinetics of Cellulase-Producing T. reesei Viscous Suspensions
(1995)Steady and dynamic shear measurements are utilized to characterize the rheological behavior of Trichoderma reesi RUT-C30 fungal suspensions during batch growth on xylose (soluble substrate) or cellulose (particulate solid ... -
Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy
(American Society for Microbiology, 2004-09-27)Previous studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) ... -
Proteome Analysis to Assess Physiological Changes in Escherichia coli Grown Under Glucose-Limited Fed-Batch Conditions
(Wiley Periodicals, 2005-09-22)Proteome analysis was used to compare global protein expression changes in Escherichia coli fermentation between exponential and glucose-limited fed-batch phase. Two-dimensional gel electrophoresis and MALDI-TOF mass ... -
The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
(Originally published by: BMC Microbiology, 2007-03-15)Background: In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the ... -
Altered structural and mechanical properties in decellularized rabbit carotid arteries
(Elsevier B.V, 2008-12-11)Recently, major achievements in creating decellularized whole tissue scaffolds have drawn considerable attention to decellularization as a promising approach for tissue engineering. Decellularized tissues are expected ... -
Microbial nar-GFP cell sensors reveal oxygen limitations in highly agitated and aerated laboratory-scale fermentors
(BioMed Central, 2009-01-15)Background: Small-scale microbial fermentations are often assumed to be homogeneous, and oxygen limitation due to inadequate micromixing is often overlooked as a potential problem. To assess the relative degree of ... -
Serial displacement chromatofocusing and its applications in multidimensional chromatography and gel electrophoresis: I. Theory and general considerations
(Elsevier B.V, 2009-02-06)The technique of “serial displacement chromatofocusing” (SDC) is investigated both theoretically and experimentally with model mixtures of proteins and peptides. The method employs a multistep, retained pH gradient formed ... -
Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds
(Elsevier B.V, 2009-03-14)We present the development and characterization of fluorescent oxygen-sensing microparticles designed for measuring oxygen concentration in microenvironments existing within standard cell culture and transparent three-dimensional ... -
Bridging the divide between neuroprosthetic design, tissue engineering and neurobiology
(Frontiers Media S.A., 2010-02-08)Neuroprosthetic devices have made a major impact in the treatment of a variety of disorders such as paralysis and stroke. However, a major impediment in the advancement of this technology is the challenge of maintaining ... -
Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties
(Elsevier B.V, 2010-04-10)Synthetic three-dimensional (3D) scaffolds for cell and tissue engineering routinely utilize peptide ligands to provide sites for cell adhesion and to promote cellular activity. Given the fact that recent studies have ... -
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties
(American Chemical Society, 2010-05-10)The objective of this work was to create three-dimensional (3D) hydrogel matrices with defined mechanical properties, as well as tunable degradability for use in applications involving protein delivery and cell encapsulation. ... -
Solute diffusion and interactions in cross-linked poly(ethylene glycol) hydrogels studied by Fluorescence Correlation Spectroscopy
(Royal Society of Chemistry, 2010-06-07)Controlled diffusion and release of soluble molecules is one of the key challenges in developing three-dimensional (3D) scaffolds for tissue engineering and drug delivery applications in part because current methods to ... -
Synthesis and Characterization of Carboxymethylcellulose-Methacrylate Hydrogel Cell Scaffolds
(MDPI, 2010-08-26)Many carbohydrates pose advantages for tissue engineering applications due to their hydrophilicity, degradability, and availability of chemical groups for modification. For example, carboxymethylcellulose (CMC) is a ... -
Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels
(Wiley Periodicals, 2010-08-27)We present a novel fully hydrophilic, hydrolytically degradable poly(ethylene glycol) (PEG) hydrogel suitable for soft tissue engineering and delivery of protein drugs. The gels were designed to overcome drawbacks associated ... -
Substrate Three-Dimensionality Induces Elemental Morphological Transformation of Sensory Neurons on a Physiologic Timescale
(Mary Ann Liebert, Inc, 2011-09-08)The natural environment of a neuron is the three-dimensional (3D) tissue. In vivo, embryonic sensory neurons transiently express a bipolar morphology with two opposing neurites before undergoing cytoplasmic and cytoskeletal ... -
Neuron Mid-Infrared Absorption Study for Direct Optical Excitation of Neurons
(IEEE, 2011-10-09)Neuron optical excitations are important for brain-circuitry explorations and sensory-neuron-stimulation applications. To optimize the stimulation, we identify neuron mid-IR absorption peaks in this study and discuss their ... -
Neuron absorption study and mid-IR optical excitations
(SPIE, 2012-02-09)Neuronal optical excitation can provide non-contacting tools to explore brain circuitry and a durable stimulation interface for cardiac pacing and visual as well as auditory sensory neuronal stimulation. To obtain accurate ... -
Fabrication of a layered microstructured polycaprolactone construct for 3-D tissue engineering
(Taylor and Francis Online, 2012-04-02)Successful artificial tissue scaffolds support regeneration by promoting cellular organization as well as appropriate mechanical and biological functionality. We have previously shown in vitro that 2-D substrates with ... -
Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures
(Wiley Periodicals, 2012-04-17)Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic ... -
Analyzing Two-Dimensional Gel Images
(Taylor and Francis Online, 2012-09-20)