Browsing UMBC Chemical, Biochemical & Environmental Engineering Department by Issue Date
Now showing items 1-20 of 38
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The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
(Originally published by: BMC Microbiology, 2007-03-15)Background: In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the ... -
Altered structural and mechanical properties in decellularized rabbit carotid arteries
(Elsevier B.V, 2008-12-11)Recently, major achievements in creating decellularized whole tissue scaffolds have drawn considerable attention to decellularization as a promising approach for tissue engineering. Decellularized tissues are expected ... -
Serial displacement chromatofocusing and its applications in multidimensional chromatography and gel electrophoresis: I. Theory and general considerations
(Elsevier B.V, 2009-02-06)The technique of “serial displacement chromatofocusing” (SDC) is investigated both theoretically and experimentally with model mixtures of proteins and peptides. The method employs a multistep, retained pH gradient formed ... -
Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds
(Elsevier B.V, 2009-03-14)We present the development and characterization of fluorescent oxygen-sensing microparticles designed for measuring oxygen concentration in microenvironments existing within standard cell culture and transparent three-dimensional ... -
Bridging the divide between neuroprosthetic design, tissue engineering and neurobiology
(Frontiers Media S.A., 2010-02-08)Neuroprosthetic devices have made a major impact in the treatment of a variety of disorders such as paralysis and stroke. However, a major impediment in the advancement of this technology is the challenge of maintaining ... -
Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties
(Elsevier B.V, 2010-04-10)Synthetic three-dimensional (3D) scaffolds for cell and tissue engineering routinely utilize peptide ligands to provide sites for cell adhesion and to promote cellular activity. Given the fact that recent studies have ... -
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties
(American Chemical Society, 2010-05-10)The objective of this work was to create three-dimensional (3D) hydrogel matrices with defined mechanical properties, as well as tunable degradability for use in applications involving protein delivery and cell encapsulation. ... -
Solute diffusion and interactions in cross-linked poly(ethylene glycol) hydrogels studied by Fluorescence Correlation Spectroscopy
(Royal Society of Chemistry, 2010-06-07)Controlled diffusion and release of soluble molecules is one of the key challenges in developing three-dimensional (3D) scaffolds for tissue engineering and drug delivery applications in part because current methods to ... -
Synthesis and Characterization of Carboxymethylcellulose-Methacrylate Hydrogel Cell Scaffolds
(MDPI, 2010-08-26)Many carbohydrates pose advantages for tissue engineering applications due to their hydrophilicity, degradability, and availability of chemical groups for modification. For example, carboxymethylcellulose (CMC) is a ... -
Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels
(Wiley Periodicals, 2010-08-27)We present a novel fully hydrophilic, hydrolytically degradable poly(ethylene glycol) (PEG) hydrogel suitable for soft tissue engineering and delivery of protein drugs. The gels were designed to overcome drawbacks associated ... -
Substrate Three-Dimensionality Induces Elemental Morphological Transformation of Sensory Neurons on a Physiologic Timescale
(Mary Ann Liebert, Inc, 2011-09-08)The natural environment of a neuron is the three-dimensional (3D) tissue. In vivo, embryonic sensory neurons transiently express a bipolar morphology with two opposing neurites before undergoing cytoplasmic and cytoskeletal ... -
Neuron absorption study and mid-IR optical excitations
(SPIE, 2012-02-09)Neuronal optical excitation can provide non-contacting tools to explore brain circuitry and a durable stimulation interface for cardiac pacing and visual as well as auditory sensory neuronal stimulation. To obtain accurate ... -
Fabrication of a layered microstructured polycaprolactone construct for 3-D tissue engineering
(Taylor and Francis Online, 2012-04-02)Successful artificial tissue scaffolds support regeneration by promoting cellular organization as well as appropriate mechanical and biological functionality. We have previously shown in vitro that 2-D substrates with ... -
Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures
(Wiley Periodicals, 2012-04-17)Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic ... -
Protein–Hydrogel Interactions in Tissue Engineering: Mechanisms and Applications
(Mary Ann Liebert, Inc, 2012-11-13)Recent advances in our understanding of the sophistication of the cellular microenvironment and the dynamics of tissue remodeling during development, disease, and regeneration have increased our appreciation of the current ... -
Spatially monitoring oxygen level in 3D microfabricated cell culture systems using optical oxygen sensing beads
(Royal Society of Chemistry, 2013-04-21)Capability of measuring and monitoring local oxygen concentration at the single cell level (tens of microns scale) is often desirable but difficult to achieve in cell culture. In this study, biocompatible oxygen sensing ... -
Biocompatible hydrogel membranes for the protection of RNA aptamer-based electrochemical sensors
(SPIE, 2013-05-31)Electrochemical-aptamer based (E-AB) sensors represent a universal specific, selective, and sensitive sensing platform for the detection of small molecule targets. Their specific detection abilities are afforded by ... -
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds as a cell delivery vehicle: Characterization of PC12 cell response
(Wiley Periodicals, 2013-06-22)The central nervous system (CNS) has a low intrinsic potential for regeneration following injury and disease, yet neural stem/progenitor cell (NPC) transplants show promise to provide a dynamic therapeutic in this complex ... -
β1-Integrin cytoskeletal signaling regulates sensory neuron response to matrix dimensionality
(Elsevier B.V, 2013-09-17)Neuronal differentiation, pathfinding and morphology are directed by biochemical cues that in vivo are presented in a complex scaffold of extracellular matrix (ECM). This microenvironment is three-dimensional (3D) and ... -
Development of chromatofocusing techniques employing mixed-mode column packings for protein separations.
(Elsevier B.V, 2013-11-06)Recent studies reported in the literature using mixed-mode chromatography (MMC) column packings have shown that multiple modes of interactions between the column packing and proteins can be usefully exploited to yield ...