Now showing items 1-2 of 2

    • Improving the recombinant human erythropoietin glycosylationusing microsome supplementation in CHO cell-free system 

      Gurramkonda, Chandrasekhar; Rao, Aniruddha; Borhani, Shayan; Pilli, Manohar; Deldari, Sevda; Ge, Xudong; Pezeshk, Niloufar; Tolosi, Michael; Kostov, Yordon; Tolosa, Leah; Frey, Douglas D.; Rao, Govind (Wiley, 2018)
      Cell-Free Protein Synthesis (CFPS) offers many advantages for the production of recombinant therapeutic proteins using the HO cell-free system. However, many complex proteins are still difficult to express using this method. ...
    • Rapid recombinant protein expression in cell-free extracts from human blood 

      Burgenson, David; Gurramkonda, Chandrasekhar; Pilli, Manohar; Ge, Xudong; Andar, Abhay; Kostov, Yordan; Tolosa, Leah; Rao, Govind (Nature, 2018-06-22)
      Several groups have recently reported on the utility of cell-free expression systems to make therapeutic proteins, most of them employing CHO or E. coli cell-free extracts. Here, we propose an alternative that uses human ...