Browsing UMBC Biological Sciences Department by Issue Date
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Specialized transducing phages for ribosomal protein genes of Escherichia coli
(National Academy of Sciences, 1975-01-01)Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein ... -
Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits
(National Academy of Sciences, 1975-07-01)This work was supported in part by the College of Agriculture and Life Sciences, University of Wisconsin, and by grants from the National Science Foundation (GB31086) and the National Institute of General Medical Sciences ... -
Mapping of ribosomal protein genes by in vitro protein synthesis using DNA fragments of lambda fus3 transducing phage DNA as templates
(1977-10-25)hfus3 (or hfus2) transducing phage stimulates the synthesis of 27 ribosomal proteins, elongation factors EF-Tu and EF-G, and RNA polymerase subunit (Y in ultraviolet-irradiated Escherichia roli cells (see a preceding ... -
Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli
(American Society for Microbiology, 1977-12)We have screened the strains with ColE1 hybrid plasmids constructed by Clarke and Carbon (Cell 9:91-99, 1976) for the presence of ribosomal ribonucleic acid (rRNA) genes on the plasmids and identified 16 strains whose ... -
Operon-specific regulation of ribosomal protein synthesis in Escherichia coli
(National Academy of Sciences, 1979-12)We have cloned a DNA fragment harboring the genes for ribosomal proteins L2, L4, and L23 on a plasmid vector that contains a lac operator and promoter. The cloned ribosomal protein genes are now under the control of lacOP. ... -
High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli
(American Society for Microbiology (ASM), 1981-01)We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% ... -
Fluorescence of crayfish metarhodopsin studied in single rhabdoms
(Elsevier Inc., 1981-09)Isolated photoreceptor organelles (rhabdoms) from eyes of crayfish (Procambarus. Orconectes) were examined on a microscope system designed for quantitative measurements of fluorescent. Although fully dark-adapted rhabdoms ... -
Oversynthesis of elongation factors G and Tu in Escherichia coli
(American Society for Microbiology (ASM), 1982-02)We induced the oversynthesis of elongation factors Tu and G by using multicopy plasmids carrying the structural genes for these proteins under the control of the lac operator-promoter. We found no evidence that accumulation ... -
Photosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsin
(Rockefeller University Press, 1982-02-01)Discrepancies exist among spectral measurements of sensitivity of crayfish photoreceptors, their absorption in situ, and the number and absorption spectra of crayfish photopigments that are extracted by digitonin solutions. ... -
Regulation of nitrogen catabolic enzymes in Bacillus spp
(American Society for Microbiology, 1982-08)The levels of the inducible nitrogen catabolic enzymes arginase (L-arginine amidinohydrolase, EC 3.5.3.1) and alanine dehydrogenase (L-alanine:NAD+ oxidoreductase [deaminating], EC 1.4.1.1) from Bacillus licheniformis and ... -
Control of diel vertical migration: Photoresponses of a larval crustacean
(American Society of Limnology and Oceanography, Inc, 1984-01)The lower threshold for phototaxis of dark‐adapted larvae of the crab Rhithropanopeus harrisii is 10⁻⁷ W·m⁻² at 500‐nm light. This threshold does not change throughout development. In the Newport River estuary (North ... -
The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.
(Oxford University Press, 1984-02-24)We have determined the nucleotide sequence of the Escherichia coli fus gene, which codes for elongation factor G. The protein product of the sequenced gene contains 703 amino acids, with a predicted molecular weight of ... -
Role of attenuation in growth rate-dependent regulation of the S10 r-protein operon of E. coli
(The European Molecular Biology Organization, 1984-07)We have investigated the transcription of the 11 gene S10 ribosomal protein operon of Escherichia coli under various growth conditions. The differential synthesis rate of structural gene message increases 2- to 2.5-fold ... -
Dark regeneration of rhodopsin in crayfish photoreceptors
(The Rockefeller University Press, 1984-07-01)The eyes of crayfish were exposed to lights of known spectral composition, and the course of regeneration was followed in the dark by measuring the content of rhodopsin and metarhodopsin in single rhabdoms isolated at ... -
Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis
(American Society for Microbiology, 1984-08)The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive ,β-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. -
Purification and properties of glutamate synthase from Bacillus licheniformis
(American Society for Microbiology, 1984-11)Glutamate synthase [L-glutamate:NADP+ oxidoreductase (transaminating); EC 1.4.1.13] (GItS) was purified to homogeneity from BaciUus licheniformis A5. The native enzyme had a molecular weight of approximately 220,000 and ... -
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product
(American Society for Microbiology, 1985-05)Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than in the same strain under ... -
Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli
(Oxford University Press, 1985-06-11)In Escherichia coli some 19 transcription units encoding the 52 ribosomal proteins are scattered throughout the genome. One of the units, the alpha operon, encodes genes for the ribosomal proteins S13, S11, S4 and L17 as ... -
Transcriptional control of the S10 ribosomal protein operon of Escherichia coli after a shift to higher temperature
(American Society for Microbiology, 1985-07)In the 5 to 10 min immediately following a shift from 30 to 42 degrees C, the differential synthesis rates of ribosomal proteins encoded by the 11-gene S10 operon are transiently decreased. This effect results largely from ... -
Precursor for elongation factor Tu from Escherichia coli
(American Society for Microbiology, 1986-02)The tufA gene, one of two genes in Escherichia coli encoding elongation factor Tu (EF-Tu), was cloned into a ColE1-derived plasmid downstream of the lac promoter-operator. In cells carrying this plasmid, the synthesis of ...