Toxoplasma gondii: further studies on the subpellicular network
| dc.contributor.author | Lemgruber, Leandro | |
| dc.contributor.author | Kloetzel, John A | |
| dc.contributor.author | Souza, Wanderley de | |
| dc.contributor.author | Vommaro, Rossiane C | |
| dc.date.accessioned | 2021-03-19T16:34:55Z | |
| dc.date.available | 2021-03-19T16:34:55Z | |
| dc.date.issued | 2009-08 | |
| dc.description.abstract | The association of the pellicle with cytoskeletal elements in Toxoplasma gondii allows this parasite to maintain its mechanical integrity and makes possible its gliding motility and cell invasion. The inner membrane complex (IMC) resembles the flattened membrane sacs observed in free-living protozoa and these sacs have been found to associate with cytoskeletal proteins such as articulins. We used immunofluorescence microscopy to characterise the presence and distribution of plateins, a sub-family of articulins, in T. gondii tachyzoites. A dispersed labelling of the whole protozoan body was observed. Electron microscopy of detergent-extracted cells revealed the presence of a network of 10 nm filaments distributed throughout the parasite. These filaments were labelled with anti-platein antibodies. Screening the sequenced T. gondii genome, we obtained the sequence of an IMC predicted protein with 25% identity and 42% similarity to the platein isoform alpha 1 present in Euplotes aediculatus, but with 42% identity and 55% similarity to that found in Euglena gracilis, suggesting strong resemblance to articulins. | en_US |
| dc.description.sponsorship | To Dr. André Jeovanio-Silva and Sarah Nardeli, for helpful discussions, and to Antonio Bosco, for technical support. | en_US |
| dc.description.uri | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500007&lng=en&tlng=en | en_US |
| dc.format.extent | 4 pages | en_US |
| dc.genre | journal articles | en_US |
| dc.identifier | doi:10.13016/m2hcvi-zotl | |
| dc.identifier.citation | Leandro Lemgruber, John A Kloetzel, Wanderley de Souza and Rossiane C Vommaro, Toxoplasma gondii: further studies on the subpellicular network, Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 104(5): 706-709, DOI: https://doi.org/10.1590/S0074-02762009000500007 | en_US |
| dc.identifier.uri | https://doi.org/10.1590/S0074-02762009000500007 | |
| dc.identifier.uri | http://hdl.handle.net/11603/21191 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Scielo | en_US |
| dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
| dc.relation.ispartof | UMBC Biological Sciences Department Collection | |
| dc.relation.ispartof | UMBC Faculty Collection | |
| dc.rights | This item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author. | |
| dc.rights | Attribution-NonCommercial 4.0 International | * |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/deed.en | * |
| dc.title | Toxoplasma gondii: further studies on the subpellicular network | en_US |
| dc.type | Text | en_US |