Application of High Throughput Bioreactors for Subclone Selection
dc.contributor.advisor | Rao, Govind | |
dc.contributor.advisor | Moreira, Antonio | |
dc.contributor.author | Drew, Jessica Lauren | |
dc.contributor.department | Chemical, Biochemical & Environmental Engineering | |
dc.contributor.program | Engineering | |
dc.date.accessioned | 2015-10-14T03:13:27Z | |
dc.date.available | 2015-10-14T03:13:27Z | |
dc.date.issued | 2008-10-22 | |
dc.description.abstract | The demand for monoclonal antibodies in pharmaceutical drug production requires the highest technology be invested in obtaining a stable, high producing cell line. Currently the most common method of selection is by limiting dilution cloning, done in well plates. The highest producing cell is chosen after samples from the stationary plates have been analyzed for antibody production. The selection is based on stationary culture, even though after scale-up cells will grow in a stirred environment. This research investigates a way to test multiple clones in a stirred environment by using high throughput bioreactors (HTBRs) in the early stages of clone selection. It has been found that simply selecting subclones based on results from stationary culture could result in the chance of missing even higher producing clones. Instead, choosing a clone after analyzing its performance in a stirred environment is an improved method to select a cell line for further scale-up. | |
dc.format | application/pdf | |
dc.genre | theses | |
dc.identifier | doi:10.13016/M2609X | |
dc.identifier.other | 1261 | |
dc.identifier.uri | http://hdl.handle.net/11603/1057 | |
dc.language | en | |
dc.relation.isAvailableAt | The University of Maryland, Baltimore County (UMBC) | |
dc.relation.ispartof | UMBC Theses and Dissertations Collection | |
dc.relation.ispartof | UMBC Graduate School Collection | |
dc.relation.ispartof | UMBC Student Collection | |
dc.relation.ispartof | UMBC Chemical, Biochemical & Environmental Engineering Department Collection | |
dc.rights | This item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please see http://aok.lib.umbc.edu/specoll/repro.php or contact Special Collections at speccoll(at)umbc.edu. | |
dc.source | Original File Name: umi-umbc-1261.pdf | |
dc.subject | Engineering, Chemical(0542) | |
dc.subject | subcloning | |
dc.subject | mammalian cell culture | |
dc.subject | improved protein productivity | |
dc.subject | miniature bioreactors | |
dc.subject | limiting dilution cloning | |
dc.title | Application of High Throughput Bioreactors for Subclone Selection | |
dc.type | Text | |
dcterms.accessRights | Access limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission. |
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