Application of High Throughput Bioreactors for Subclone Selection

dc.contributor.advisorRao, Govind
dc.contributor.advisorMoreira, Antonio
dc.contributor.authorDrew, Jessica Lauren
dc.contributor.departmentChemical, Biochemical & Environmental Engineering
dc.description.abstractThe demand for monoclonal antibodies in pharmaceutical drug production requires the highest technology be invested in obtaining a stable, high producing cell line. Currently the most common method of selection is by limiting dilution cloning, done in well plates. The highest producing cell is chosen after samples from the stationary plates have been analyzed for antibody production. The selection is based on stationary culture, even though after scale-up cells will grow in a stirred environment. This research investigates a way to test multiple clones in a stirred environment by using high throughput bioreactors (HTBRs) in the early stages of clone selection. It has been found that simply selecting subclones based on results from stationary culture could result in the chance of missing even higher producing clones. Instead, choosing a clone after analyzing its performance in a stirred environment is an improved method to select a cell line for further scale-up.
dc.relation.isAvailableAtThe University of Maryland, Baltimore County (UMBC)
dc.relation.ispartofUMBC Theses and Dissertations Collection
dc.relation.ispartofUMBC Graduate School Collection
dc.relation.ispartofUMBC Student Collection
dc.relation.ispartofUMBC Chemical, Biochemical & Environmental Engineering Department Collection
dc.rightsThis item may be protected under Title 17 of the U.S. Copyright Law. It is made available by UMBC for non-commercial research and education. For permission to publish or reproduce, please see or contact Special Collections at speccoll(at)
dc.sourceOriginal File Name: umi-umbc-1261.pdf
dc.subjectEngineering, Chemical(0542)
dc.subjectmammalian cell culture
dc.subjectimproved protein productivity
dc.subjectminiature bioreactors
dc.subjectlimiting dilution cloning
dc.titleApplication of High Throughput Bioreactors for Subclone Selection
dcterms.accessRightsAccess limited to the UMBC community. Item may possibly be obtained via Interlibrary Loan through a local library, pending author/copyright holder's permission.


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