Zengel, J.M.Archer, R.H.Freedman, L.P.Lindahl, L.2019-06-042019-06-041984-07J M Zengel, R H Archer, L P Freedman, and L Lindahl, Role of attenuation in growth rate-dependent regulation of the S10 r-protein operon of E. coli, EMBO J. 1984 Jul; 3(7): 1561–1565, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC557559/http://hdl.handle.net/11603/14014We have investigated the transcription of the 11 gene S10 ribosomal protein operon of Escherichia coli under various growth conditions. The differential synthesis rate of structural gene message increases 2- to 2.5-fold immediately after a shift-up from glycerol minimal medium to glucose plus amino acids. After the initial increase, the transcription rate goes through several oscillations before reaching the new steady-state rate. By comparing the rates of transcription of leader and structural genes, we conclude that these oscillations are due predominantly to changes in the level of read-through at the S10 attenuator. This regulation of attenuation can account for most of the variations in protein synthesis from the S10 operon after a shift. We also measured the level of read-through in cells growing exponentially in different growth media. Over a 2.5-fold range in growth rates, the read-through changed less than 50%. Thus, regulation of attenuation cannot explain the growth-dependent regulation of ribosomal protein synthesis during steady-state growth. Apparently, additional mechanisms are required to control the expression of the S10 operon in exponentially growing cells.5 pagesen-USThis item is likely protected under Title 17 of the U.S. Copyright Law. Unless on a Creative Commons license, for uses protected by Copyright Law, contact the copyright holder or the author.attenuationE. coliribosomesshift-uptranscriptionText